Restriction map for
pFN21AE2634
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AccI AccII AccIII
AgeI AscI AseI AsnI Asp718I
AspI AvrII BbeI BbrPI BglII
BlnI BsaAI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoT22I FseI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 862 AACGTT AA^CGTT
AflII 3052 CTTAAG C^TTAAG
AflIII 1581 ACATGT A^C[GA][CT]GT
AhdI 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 337 GTGCAC G^TGCAC
Aor51HI 3659 AGCGCT AGC^GCT
ApaLI 337 GTGCAC G^TGCAC
Asp700I 1168 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2737 TGCGCA TGC^GCA
BamHI 260 GGATCC G^GATCC
BanI 3575 GGCACC G^G[CT][GA]CC
BciVI 3945 GGATAC GTATCC(6/5)
BfrI 3052 CTTAAG C^TTAAG
BsaBI 4128 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3822 ACCGGA [AT]^CCGG[AT]
BsmI 3057 GAATGC GAATGC(1/-1)
BspHI 374 TCATGA T^CATGA
BspLU11I 1581 ACATGT A^CATGT
BsrDI 2538 GCAATG GCAATG(2/0)
BssSI 1437 CACGAG CACGAG(-5/-1)
BstYI 260 GGATCC [GA]^GATC[CT]
Eam1105I 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3659 AGCGCT AGC^GCT
EcoRI 3475 GAATTC G^AATTC
EcoRV 3841 GATATC GAT^ATC
FspI 2737 TGCGCA TGC^GCA
HincII 1258 GTCAAC GT[CT]^[GA]AC
HindII 1258 GTCAAC GT[CT]^[GA]AC
HindIII 1216 AAGCTT A^AGCTT
MamI 4128 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 260 GGATCC [GA]^GATC[CT]
NcoI 1118 CCATGG C^CATGG
Psp1406I 862 AACGTT AA^CGTT
RcaI 374 TCATGA T^CATGA
SmaI 1322 CCCGGG CCC^GGG
SphI 755 GCATGC GCATG^C
XhoII 260 GGATCC [GA]^GATC[CT]
XmaCI 1322 CCCGGG C^CCGGG
XmaI 1322 CCCGGG C^CCGGG
XmnI 1168 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 492 GGCGTC G[GA]^CG[CT]C
2803 GACGCC
AfaI 402 GTAC GT^AC
4048 GTAC
BalI 233 TGGCCA TGG^CCA
523 TGGCCA
BcgI 1659 CGAGCGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3924 CGACCCAAATGC
BclI 165 TGATCA T^GATCA
4040 TGATCA
BsaHI 492 GGCGTC G[GA]^CG[CT]C
2803 GACGCC
BsaI 1555 GAGACC GGTCTC(1/5)
1526 GGTCTC
BsrBI 1660 GAGCGG CCGCTC(-3/-3)
3415 GAGCGG
BstAPI 1419 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1917 GCAAGCCCTGC
BstEII 909 GGTCACC G^GT[ACGT]ACC
2020 GGTGACC
BstPI 909 GGTCACC G^GT[ACGT]ACC
2020 GGTGACC
EcoO65I 909 GGTCACC G^GT[ACGT]ACC
2020 GGTGACC
FbaI 165 TGATCA T^GATCA
4040 TGATCA
Hin1I 492 GGCGTC G[GA]^CG[CT]C
2803 GACGCC
MaeII 863 ACGT A^CGT
3629 ACGT
MluNI 233 TGGCCA TGG^CCA
523 TGGCCA
MscI 233 TGGCCA TGG^CCA
523 TGGCCA
MseI 2510 TTAA T^TAA
3053 TTAA
RsaI 402 GTAC GT^AC
4048 GTAC
StuI 1748 AGGCCT AGG^CCT
4152 AGGCCT
Tru9I 2510 TTAA T^TAA
3053 TTAA
XcmI 1850 CCAACAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2256 CCAACAGGGAGTTGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NheI NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfiI SmlI
Sse8387I StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI