Restriction map for
pFN21AE2697
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeIII HapII Hin1I
HpaI HpaII KasI KspI MamI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1194 GGTACC G^GTACC
AccII 1450 CGCG CG^CG
AlwNI 1505 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1472 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1194 GGTACC G^GTACC
AspHI 539 GAGCAC G[AT]GC[AT]^C
AspI 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 648 CTCGAG C^[CT]CG[GA]G
BamHI 1726 GGATCC G^GATCC
BanII 27 GGGCTC G[GA]GC[CT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BpuAI 403 GAAGAC GAAGAC(2/6)
BsaAI 1688 CACGTA [CT]AC^GT[GA]
BsgI 777 CTGCAC GTGCAG(16/14)
BsiHKAI 539 GAGCAC G[AT]GC[AT]^C
BsiYI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 648 CTCGAG C^[CT]CG[GA]G
BssSI 1879 CTCGTG CACGAG(-5/-1)
BstUI 1450 CGCG CG^CG
EcoNI 151 CCTCCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 865 CCAAGG C^C[AT][AT]GG
FauI 24 GCGGG CCCGC(4/6)
HaeII 37 GGCGCT [GA]GCGC^[CT]
HgaI 1448 GACGC GACGC(5/10)
HincII 1744 GTTGAC GT[CT]^[GA]AC
HindII 1744 GTTGAC GT[CT]^[GA]AC
HindIII 828 AAGCTT A^AGCTT
KpnI 1194 GGTACC GGTAC^C
MfeI 323 CAATTG C^AATTG
MslI 787 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 622 CAGCTG C[AC]G^C[GT]G
MunI 323 CAATTG C^AATTG
MvnI 1450 CGCG CG^CG
NdeI 544 CATATG CA^TATG
PaeR7I 648 CTCGAG C^TCGAG
PflFI 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 570 GAGTC GAGTC(4/5)
PvuII 622 CAGCTG CAG^CTG
SfcI 155 CTGTAG C^T[GA][CT]AG
SspI 718 AATATT AAT^ATT
StyI 865 CCAAGG C^C[AT][AT]GG
Tth111I 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1238 CCACTGTTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 648 CTCGAG C^TCGAG
XmnI 1472 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 1279 GTAGAC GT^[AC][GT]AC
1418 GTAGAC
AcsI 838 GAATTT [GA]^AATT[CT]
1005 AAATTC
ApoI 838 GAATTT [GA]^AATT[CT]
1005 AAATTC
AvaII 1036 GGACC G^G[AT]CC
1453 GGTCC
BanI 463 GGTGCC G^G[CT][GA]CC
1194 GGTACC
BglI 111 GCCTGATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
424 GCCAAAGAGGC
BglII 337 AGATCT A^GATCT
681 AGATCT
BpmI 1794 CTCCAG CTGGAG(16/14)
625 CTGGAG
BsaJI 742 CCTGGG C^C[ACGT][ACGT]GG
865 CCAAGG
BsmBI 1520 GAGACG CGTCTC(1/5)
1586 GAGACG
BspMI 357 ACCTGC ACCTGC(4/8)
775 ACCTGC
BssKI 742 CCTGG ^CC[ACGT]GG
1456 CCTGG
BstNI 742 CCTGG CC^[AT]GG
1456 CCTGG
Cfr13I 1036 GGACC G^G[ACGT]CC
1453 GGTCC
DraI 448 TTTAAA TTT^AAA
1269 TTTAAA
EcoRII 742 CCTGG ^CC[AT]GG
1456 CCTGG
MvaI 742 CCTGG CC^[AT]GG
1456 CCTGG
NspI 292 GCATGT [GA]CATG^[CT]
557 ACATGC
Sau96I 1036 GGACC G^G[ACGT]CC
1453 GGTCC
ScrFI 742 CCTGG CC^[ACGT]GG
1456 CCTGG
SmlI 648 CTCGAG C^T[CT][GA]AG
1174 CTCAAG
XbaI 762 TCTAGA T^CTAGA
1342 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AviII
BbvI BfaI BmyI BseRI BsmAI
Bsp1286I BspHI BsrI BstAPI BstF5I
BstYI Cac8I CfoI DdeI DpnII
EarI Fnu4HI FokI FspI HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV RcaI RsaI Sau3AI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII