Restriction map for
pFN21AE2702
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII HindIII
HpaI HpaII KasI KpnI MamI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 46 GGCGTC G[GA]^CG[CT]C
AocI 847 CCTCAGG CC^T[ACGT]AGG
AseI 231 ATTAAT AT^TAAT
AsnI 231 ATTAAT AT^TAAT
AspHI 681 GAGCAC G[AT]GC[AT]^C
BamHI 403 GGATCC G^GATCC
BanI 339 GGCACC G^G[CT][GA]CC
BciVI 588 GTATCC GTATCC(6/5)
BfaI 562 CTAG C^TAG
BmyI 681 GAGCAC G[AGT]GC[ACT]^C
BsaHI 46 GGCGTC G[GA]^CG[CT]C
BsaI 305 GAGACC GGTCTC(1/5)
BseRI 712 CTCCTC GAGGAG(10/8)
BsgI 116 GTGCAG GTGCAG(16/14)
BsiEI 128 CGGCCG CG[GA][CT]^CG
BsiHKAI 681 GAGCAC G[AT]GC[AT]^C
BsmI 1173 GCATTC GAATGC(1/-1)
Bsp1286I 681 GAGCAC G[AGT]GC[ACT]^C
BspHI 311 TCATGA T^CATGA
BsrBI 1228 CCGCTC CCGCTC(-3/-3)
BssHII 36 GCGCGC G^CGCGC
BstXI 97 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 403 GGATCC [GA]^GATC[CT]
Bsu36I 847 CCTCAGG CC^T[ACGT]AGG
EagI 128 CGGCCG C^GGCCG
EclXI 128 CGGCCG C^GGCCG
Eco52I 128 CGGCCG C^GGCCG
Eco81I 847 CCTCAGG CC^T[ACGT]AGG
FauI 43 GCGGG CCCGC(4/6)
HaeII 859 AGCGCC [GA]GCGC^[CT]
HgaI 47 GCGTC GACGC(5/10)
Hin1I 46 GGCGTC G[GA]^CG[CT]C
HincII 826 GTCAAC GT[CT]^[GA]AC
HindII 826 GTCAAC GT[CT]^[GA]AC
KspI 41 CCGCGG CCGC^GG
MaeI 562 CTAG C^TAG
MaeII 512 ACGT A^CGT
MfeI 536 CAATTG C^AATTG
MflI 403 GGATCC [GA]^GATC[CT]
MslI 98 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 536 CAATTG C^AATTG
NcoI 97 CCATGG C^CATGG
NspI 153 GCATGC [GA]CATG^[CT]
PflMI 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 951 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 231 ATTAAT AT^TAAT
RcaI 311 TCATGA T^CATGA
SacII 41 CCGCGG CCGC^GG
SapI 1115 GAAGAGC GCTCTTC(1/4)
SphI 153 GCATGC GCATG^C
SspI 228 AATATT AAT^ATT
TaqI 1067 TCGA T^CGA
TthHB8I 1067 TCGA T^CGA
Van91I 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 403 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 75 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1279 CAGCAGCTG
BpmI 81 CTGGAG CTGGAG(16/14)
889 CTGGAG
BsmFI 657 GGGAC GGGAC(10/14)
468 GTCCC
DsaI 41 CCGCGG C^C[GA][CT]GG
97 CCATGG
EaeI 128 CGGCCG [CT]^GGCC[GA]
1160 TGGCCG
MaeIII 133 GTCAC ^GT[ACGT]AC
1034 GTGAC
PstI 122 CTGCAG CTGCA^G
1248 CTGCAG
ScaI 893 AGTACT AGT^ACT
1070 AGTACT
SfaNI 939 GATGC GCATC(5/9)
70 GCATC
SfcI 122 CTGCAG C^T[GA][CT]AG
1248 CTGCAG
TfiI 372 GATTC G^A[AT]TC
967 GAATC
Tsp45I 133 GTCAC ^GT[GC]AC
1034 GTGAC
Tsp509I 464 AATT ^AATT
537 AATT
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SmlI StyI Tru9I
TseI TspRI