Restriction map for
pFN21AE2774
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BalI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraIII EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1279 CGCG CG^CG
AflIII 524 ACATGT A^C[GA][CT]GT
AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1690 CCTCAGG CC^T[ACGT]AGG
Aor51HI 27 AGCGCT AGC^GCT
ApaI 1548 GGGCCC GGGCC^C
AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 436 GGCGCC GGCGC^C
BbrPI 774 CACGTG CAC^GTG
BbsI 958 GAAGAC GAAGAC(2/6)
BciVI 1736 GTATCC GTATCC(6/5)
BclI 2090 TGATCA T^GATCA
BfaI 1267 CTAG C^TAG
BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 958 GAAGAC GAAGAC(2/6)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsmI 1600 GAATGC GAATGC(1/-1)
BspLU11I 524 ACATGT A^CATGT
BsrBI 1971 GAGCGG CCGCTC(-3/-3)
BsrDI 270 CATTGC GCAATG(2/0)
BstUI 1279 CGCG CG^CG
Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG
DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 669 CCGTGG C^C[GA][CT]GG
EagI 17 CGGCCG C^GGCCG
Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2018 CTCTTC CTCTTC(1/4)
EclXI 17 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
Eco81I 1690 CCTCAGG CC^T[ACGT]AGG
EcoRI 293 GAATTC G^AATTC
FbaI 2090 TGATCA T^GATCA
KasI 436 GGCGCC G^GCGCC
Ksp632I 2018 CTCTTC CTCTTC(1/4)
MaeI 1267 CTAG C^TAG
MvnI 1279 CGCG CG^CG
NaeI 1837 GCCGGC GCC^GGC
NarI 436 GGCGCC GG^CGCC
NgoMIV 1837 GCCGGC G^CCGGC
NspI 524 ACATGT [GA]CATG^[CT]
PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PpuMI 1444 GGGACCT [GA]G^G[AT]CC[CT]
SfoI 436 GGCGCC GGC^GCC
SmaI 728 CCCGGG CCC^GGG
SmlI 1357 CTCAAG C^T[CT][GA]AG
SspI 1063 AATATT AAT^ATT
TaqI 529 TCGA T^CGA
TfiI 1218 GATTC G^A[AT]TC
TthHB8I 529 TCGA T^CGA
Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 654 GTGCTC G[AT]GC[AT]^C
1527 GTGCTC
BamHI 1161 GGATCC G^GATCC
2009 GGATCC
BanI 436 GGCGCC G^G[CT][GA]CC
862 GGTGCC
BsaAI 774 CACGTG [CT]AC^GT[GA]
819 CACGTA
BsaWI 388 ACCGGA [AT]^CCGG[AT]
1450 TCCGGT
BsiHKAI 654 GTGCTC G[AT]GC[AT]^C
1527 GTGCTC
BsmBI 1569 CGTCTC CGTCTC(1/5)
764 GAGACG
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
BssSI 99 CACGAG CACGAG(-5/-1)
915 CTCGTG
BstAPI 1595 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1664 GCATACAGTGC
BstYI 1161 GGATCC [GA]^GATC[CT]
2009 GGATCC
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
DraI 1033 TTTAAA TTT^AAA
1921 TTTAAA
EaeI 17 CGGCCG [CT]^GGCC[GA]
1839 CGGCCA
EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1686 CCTGCCTCAGG
FauI 492 GCGGG CCCGC(4/6)
1973 GCGGG
MflI 1161 GGATCC [GA]^GATC[CT]
2009 GGATCC
PstI 310 CTGCAG CTGCA^G
710 CTGCAG
SfcI 310 CTGCAG C^T[GA][CT]AG
710 CTGCAG
XhoII 1161 GGATCC [GA]^GATC[CT]
2009 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I XcmI