Restriction map for
pFN21AE2629
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AclI AcsI AflII AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AviII BbvCI BciVI BclI BfrI
BglII BsaBI BsiWI BsmI Bsp1407I
BspLU11I BsrBI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoRI EcoT22I
FbaI FspI HindIII HpaI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2001 GGTACC G^GTACC
AccI 190 GTCTAC GT^[AC][GT]AC
AccIII 237 TCCGGA T^CCGGA
Alw44I 1993 GTGCAC G^TGCAC
ApaLI 1993 GTGCAC G^TGCAC
Asp718I 2001 GGTACC G^GTACC
AspI 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 744 CCTAGG C^CTAGG
BalI 2568 TGGCCA TGG^CCA
BbrPI 2558 CACGTG CAC^GTG
BbsI 2386 GTCTTC GAAGAC(2/6)
BcgI 1583 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 744 CCTAGG C^CTAGG
BpuAI 2386 GTCTTC GAAGAC(2/6)
BsaAI 2558 CACGTG [CT]AC^GT[GA]
BseAI 237 TCCGGA T^CCGGA
BspDI 2803 ATCGAT AT^CGAT
BspEI 237 TCCGGA T^CCGGA
BspHI 2018 TCATGA T^CATGA
BsrDI 2156 GCAATG GCAATG(2/0)
BstEII 291 GGTGACC G^GT[ACGT]ACC
BstPI 291 GGTGACC G^GT[ACGT]ACC
ClaI 2803 ATCGAT AT^CGAT
EcoO65I 291 GGTGACC G^GT[ACGT]ACC
EcoRV 841 GATATC GAT^ATC
FseI 1290 GGCCGGCC GGCCGG^CC
HincII 2494 GTTGAC GT[CT]^[GA]AC
HindII 2494 GTTGAC GT[CT]^[GA]AC
KpnI 2001 GGTACC GGTAC^C
MluNI 2568 TGGCCA TGG^CCA
MroI 237 TCCGGA T^CCGGA
MscI 2568 TGGCCA TGG^CCA
NcoI 1911 CCATGG C^CATGG
PflFI 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2558 CACGTG CAC^GTG
PmlI 2558 CACGTG CAC^GTG
RcaI 2018 TCATGA T^CATGA
SapI 1878 GAAGAGC GCTCTTC(1/4)
SfiI 1260 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2297 GCATGC GCATG^C
StuI 531 AGGCCT AGG^CCT
TfiI 1061 GAATC G^A[AT]TC
Tth111I 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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ApaI 69 GGGCCC GGGCC^C
2809 GGGCCC
BsaI 1507 GAGACC GGTCTC(1/5)
2638 GAGACC
BsaWI 237 TCCGGA [AT]^CCGG[AT]
1706 ACCGGA
BssSI 1633 CTCGTG CACGAG(-5/-1)
2564 CTCGTG
BstAPI 1896 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2646 GCAGGCCCTGC
EarI 64 GAAGAG CTCTTC(1/4)
1878 GAAGAG
Ksp632I 64 GAAGAG CTCTTC(1/4)
1878 GAAGAG
MaeIII 292 GTGAC ^GT[ACGT]AC
581 GTGAC
MslI 1906 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2656 CACGACCGTG
NspI 368 ACATGC [GA]CATG^[CT]
2297 GCATGC
PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT]
53 AGGACCT
SacI 2398 GAGCTC GAGCT^C
2482 GAGCTC
SexAI 1997 ACCAGGT A^CC[AT]GGT
2207 ACCTGGT
SmlI 1762 CTCAAG C^T[CT][GA]AG
2401 CTCAAG
Tsp45I 292 GTGAC ^GT[GC]AC
581 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BamHI BanI BanII BbeI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfoI
SmaI Sse8387I StyI TaqI TseI
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI