Restriction map for
pFN21AE2624
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StyI SwaI TfiI Tru9I Tsp45I
TspRI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 562 GAATTC [GA]^AATT[CT]
AluI 608 AGCT AG^CT
ApoI 562 GAATTC [GA]^AATT[CT]
AscI 173 GGCGCGCC GG^CGCGCC
AspHI 661 GTGCTC G[AT]GC[AT]^C
AvaI 774 CCCGAG C^[CT]CG[GA]G
BfaI 158 CTAG C^TAG
BglI 4 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 262 CTGGAG CTGGAG(16/14)
BsaI 619 GAGACC GGTCTC(1/5)
BsiHKAI 661 GTGCTC G[AT]GC[AT]^C
BsmAI 619 GAGAC GTCTC(1/5)
BsmI 612 GCATTC GAATGC(1/-1)
BsoBI 774 CCCGAG C^[CT]CG[GA]G
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BstF5I 533 GGATG GGATG(2/0)
DpnII 673 GATC ^GATC
EarI 634 CTCTTC CTCTTC(1/4)
EcoRI 562 GAATTC G^AATTC
FokI 533 GGATG GGATG(9/13)
HincII 373 GTCAAC GT[CT]^[GA]AC
HindII 373 GTCAAC GT[CT]^[GA]AC
HinfI 313 GAGTC G^A[ACGT]TC
HphI 665 TCACC GGTGA(8/7)
Ksp632I 634 CTCTTC CTCTTC(1/4)
KspI 5 CCGCGG CCGC^GG
MaeI 158 CTAG C^TAG
MboI 673 GATC ^GATC
NdeII 673 GATC ^GATC
PflMI 750 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 313 GAGTC GAGTC(4/5)
PvuII 607 CAGCTG CAG^CTG
SacII 5 CCGCGG CCGC^GG
SapI 633 GCTCTTC GCTCTTC(1/4)
Sau3AI 673 GATC ^GATC
SbfI 134 CCTGCAGG CCTGCA^GG
SfaNI 534 GATGC GCATC(5/9)
Sse8387I 134 CCTGCAGG CCTGCA^GG
StuI 131 AGGCCT AGG^CCT
Tsp509I 563 AATT ^AATT
Van91I 750 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 399 GTAC GT^AC
648 GTAC
BsiEI 166 CGGCCG CG[GA][CT]^CG
480 CGGCCG
BsrFI 294 GCCGGC [GA]^CCGG[CT]
576 GCCGGC
BssHII 107 GCGCGC G^CGCGC
174 GCGCGC
BstAPI 330 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
702 GCAGGTCTTGC
Cfr10I 294 GCCGGC [GA]^CCGG[CT]
576 GCCGGC
DdeI 16 CTGAG C^T[ACGT]AG
739 CTGAG
DsaI 5 CCGCGG C^C[GA][CT]GG
354 CCGTGG
EagI 166 CGGCCG C^GGCCG
480 CGGCCG
EclXI 166 CGGCCG C^GGCCG
480 CGGCCG
Eco52I 166 CGGCCG C^GGCCG
480 CGGCCG
EcoNI 429 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
738 CCTGAGCCAGG
MaeII 371 ACGT A^CGT
494 ACGT
MboII 720 GAAGA GAAGA(8/7)
635 TCTTC
NaeI 294 GCCGGC GCC^GGC
576 GCCGGC
NgoMIV 294 GCCGGC G^CCGGC
576 GCCGGC
PstI 135 CTGCAG CTGCA^G
454 CTGCAG
RsaI 399 GTAC GT^AC
648 GTAC
SfcI 135 CTGCAG C^T[GA][CT]AG
454 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AlwNI ApaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIV PpuMI Sau96I ScrFI
SfoI TaqI TseI TthHB8I