Restriction map for
pFN21AE2578
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BbrPI BfrI
BlnI BsiWI BsmBI BsmI Bsp1407I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KpnI Ksp632I
MfeI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SapI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 212 TCCGGA T^CCGGA
AcsI 2331 GAATTT [GA]^AATT[CT]
ApoI 2331 GAATTT [GA]^AATT[CT]
Asp700I 384 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1188 TGCGCA TGC^GCA
BamHI 1995 GGATCC G^GATCC
BciVI 276 GGATAC GTATCC(6/5)
BclI 1911 TGATCA T^GATCA
BlpI 2737 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2737 GCTCAGC GC^T[ACGT]AGC
BsaBI 1266 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 212 TCCGGA T^CCGGA
BsiEI 3369 CGACCG CG[GA][CT]^CG
BspDI 1894 ATCGAT AT^CGAT
BspEI 212 TCCGGA T^CCGGA
BspHI 1202 TCATGA T^CATGA
BspLU11I 2279 ACATGT A^CATGT
BstEII 3381 GGTGACC G^GT[ACGT]ACC
BstPI 3381 GGTGACC G^GT[ACGT]ACC
CelII 2737 GCTCAGC GC^T[ACGT]AGC
ClaI 1894 ATCGAT AT^CGAT
EcoO65I 3381 GGTGACC G^GT[ACGT]ACC
FbaI 1911 TGATCA T^GATCA
FspI 1188 TGCGCA TGC^GCA
HincII 2751 GTCGAC GT[CT]^[GA]AC
HindII 2751 GTCGAC GT[CT]^[GA]AC
KspI 963 CCGCGG CCGC^GG
MamI 1266 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1616 ACGCGT A^CGCGT
MroI 212 TCCGGA T^CCGGA
PflFI 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1202 TCATGA T^CATGA
SacI 1216 GAGCTC GAGCT^C
SacII 963 CCGCGG CCGC^GG
SalI 2751 GTCGAC G^TCGAC
SexAI 3398 ACCAGGT A^CC[AT]GGT
Tth111I 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 384 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1616 ACGCGT A^C[GA][CT]GT
2279 ACATGT
Alw44I 1182 GTGCAC G^TGCAC
1438 GTGCAC
AlwNI 1192 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1564 CAGTACCTG
ApaLI 1182 GTGCAC G^TGCAC
1438 GTGCAC
BcgI 996 GCATGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1707 GCAGCGGACTCG
BglII 1106 AGATCT A^GATCT
1271 AGATCT
BsaAI 637 TACGTG [CT]AC^GT[GA]
1382 TACGTG
BsaI 958 GAGACC GGTCTC(1/5)
2635 GAGACC
BsaWI 212 TCCGGA [AT]^CCGG[AT]
2831 ACCGGA
BsrFI 596 ACCGGC [GA]^CCGG[CT]
1937 GCCGGT
Cfr10I 596 ACCGGC [GA]^CCGG[CT]
1937 GCCGGT
EcoNI 2403 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3393 CCTGTACCAGG
MseI 833 TTAA T^TAA
2335 TTAA
PpuMI 2565 GGGTCCC [GA]G^G[AT]CC[CT]
3362 GGGTCCC
ScaI 1979 AGTACT AGT^ACT
2441 AGTACT
SfiI 1525 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1689 GGCCATGCTGGCC
SmaI 1316 CCCGGG CCC^GGG
2885 CCCGGG
SmlI 1624 CTCAAG C^T[CT][GA]AG
1969 CTTGAG
SphI 767 GCATGC GCATG^C
2733 GCATGC
StuI 947 AGGCCT AGG^CCT
1790 AGGCCT
Tru9I 833 TTAA T^TAA
2335 TTAA
XcmI 3188 CCATGCGGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3243 CCAGATGAAGATTGG
XmaCI 1316 CCCGGG C^CCGGG
2885 CCCGGG
XmaI 1316 CCCGGG C^CCGGG
2885 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII