Restriction map for
pFN21AE9667
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AvrII BclI
BlnI BsgI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco47III Eco81I EcoRV
EcoT22I FbaI FseI HindIII HpaI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SbfI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 3421 GGTACC G^GTACC
AccI 2239 GTCGAC GT^[AC][GT]AC
AccIII 2248 TCCGGA T^CCGGA
AcyI 2905 GGCGCC G[GA]^CG[CT]C
AflII 121 CTTAAG C^TTAAG
AlwNI 391 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 3421 GGTACC G^GTACC
AviII 2317 TGCGCA TGC^GCA
BamHI 802 GGATCC G^GATCC
BbeI 2905 GGCGCC GGCGC^C
BbrPI 338 CACGTG CAC^GTG
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
BcgI 3071 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 121 CTTAAG C^TTAAG
BglI 2026 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 717 AGATCT A^GATCT
BlpI 2538 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2538 GCTGAGC GC^T[ACGT]AGC
BsaBI 1086 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2905 GGCGCC G[GA]^CG[CT]C
BseAI 2248 TCCGGA T^CCGGA
BsmI 3071 GCATTC GAATGC(1/-1)
BspEI 2248 TCCGGA T^CCGGA
BspMI 2823 ACCTGC ACCTGC(4/8)
BstAPI 2350 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2538 GCTGAGC GC^T[ACGT]AGC
DraI 1603 TTTAAA TTT^AAA
EagI 2592 CGGCCG C^GGCCG
EclXI 2592 CGGCCG C^GGCCG
Eco52I 2592 CGGCCG C^GGCCG
FspI 2317 TGCGCA TGC^GCA
HaeII 2905 GGCGCC [GA]GCGC^[CT]
HgaI 3374 GCGTC GACGC(5/10)
Hin1I 2905 GGCGCC G[GA]^CG[CT]C
KasI 2905 GGCGCC G^GCGCC
KpnI 3421 GGTACC GGTAC^C
KspI 7 CCGCGG CCGC^GG
MamI 1086 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2248 TCCGGA T^CCGGA
NarI 2905 GGCGCC GG^CGCC
NdeI 1053 CATATG CA^TATG
PmaCI 338 CACGTG CAC^GTG
PmlI 338 CACGTG CAC^GTG
PpuMI 2470 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3369 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2152 GAGCTC GAGCT^C
SacII 7 CCGCGG CCGC^GG
SalI 2239 GTCGAC G^TCGAC
ScaI 228 AGTACT AGT^ACT
SexAI 1407 ACCAGGT A^CC[AT]GGT
SfoI 2905 GGCGCC GGC^GCC
SmaI 3461 CCCGGG CCC^GGG
SspI 961 AATATT AAT^ATT
StuI 3603 AGGCCT AGG^CCT
SwaI 1602 ATTTAAAT ATTT^AAAT
XmaCI 3461 CCCGGG C^CCGGG
XmaI 3461 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AgeI 162 ACCGGT A^CCGGT
2546 ACCGGT
Asp700I 715 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3588 GAACTACTTC
AspI 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACTGTGTC
BalI 1353 TGGCCA TGG^CCA
1667 TGGCCA
BanI 2905 GGCGCC G^G[CT][GA]CC
3421 GGTACC
BciVI 31 GGATAC GTATCC(6/5)
1394 GTATCC
BfaI 1544 CTAG C^TAG
1708 CTAG
BsaAI 338 CACGTG [CT]AC^GT[GA]
3060 TACGTG
BsiEI 2592 CGGCCG CG[GA][CT]^CG
2931 CGACCG
BsmBI 1897 CGTCTC CGTCTC(1/5)
3577 GAGACG
BsrBI 1124 CCGCTC CCGCTC(-3/-3)
2205 CCGCTC
BsrFI 162 ACCGGT [GA]^CCGG[CT]
2546 ACCGGT
BssSI 2260 CACGAG CACGAG(-5/-1)
2155 CTCGTG
Cfr10I 162 ACCGGT [GA]^CCGG[CT]
2546 ACCGGT
DraIII 335 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1406 CACCAGGTG
EcoNI 88 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
969 CCTTACCAAGG
EcoRI 1891 GAATTC G^AATTC
2227 GAATTC
MaeI 1544 CTAG C^TAG
1708 CTAG
MluNI 1353 TGGCCA TGG^CCA
1667 TGGCCA
MscI 1353 TGGCCA TGG^CCA
1667 TGGCCA
NcoI 2060 CCATGG C^CATGG
2211 CCATGG
PflFI 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACTGTGTC
PinAI 162 ACCGGT A^CCGGT
2546 ACCGGT
SapI 442 GAAGAGC GCTCTTC(1/4)
1836 GAAGAGC
SgrAI 161 CACCGGTG C[GA]^CCGG[CT]G
2545 CACCGGTG
Tth111I 180 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACTGTGTC
XmnI 715 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3588 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII