Restriction map for
pFN21AE2565
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoRV FbaI FseI FspI HgaI
HindIII HpaI KpnI KspI MamI
MluI MroI MvnI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 304 GAATTC [GA]^AATT[CT]
AcyI 3349 GGCGCC G[GA]^CG[CT]C
AflIII 1514 ACATGT A^C[GA][CT]GT
Alw44I 2948 GTGCAC G^TGCAC
ApaLI 2948 GTGCAC G^TGCAC
ApoI 304 GAATTC [GA]^AATT[CT]
Asp700I 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3214 GGATCC G^GATCC
BbeI 3349 GGCGCC GGCGC^C
BbvCI 637 CCTCAGC CCTCAGC(-5/-2)
BglII 2392 AGATCT A^GATCT
BlpI 2757 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2757 GCTCAGC GC^T[ACGT]AGC
BsaHI 3349 GGCGCC G[GA]^CG[CT]C
BsiEI 1913 CGGCCG CG[GA][CT]^CG
BspHI 1862 TCATGA T^CATGA
BspLU11I 1514 ACATGT A^CATGT
BsrBI 2805 CCGCTC CCGCTC(-3/-3)
BstAPI 3244 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3403 GGTGACC G^GT[ACGT]ACC
BstPI 3403 GGTGACC G^GT[ACGT]ACC
CelII 2757 GCTCAGC GC^T[ACGT]AGC
DrdI 2547 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1913 CGGCCG C^GGCCG
EclXI 1913 CGGCCG C^GGCCG
Eco52I 1913 CGGCCG C^GGCCG
EcoO65I 3403 GGTGACC G^GT[ACGT]ACC
EcoRI 304 GAATTC G^AATTC
EcoT22I 23 ATGCAT ATGCA^T
Hin1I 3349 GGCGCC G[GA]^CG[CT]C
HincII 1967 GTCAAC GT[CT]^[GA]AC
HindII 1967 GTCAAC GT[CT]^[GA]AC
KasI 3349 GGCGCC G^GCGCC
MfeI 2939 CAATTG C^AATTG
MunI 2939 CAATTG C^AATTG
NarI 3349 GGCGCC GG^CGCC
NcoI 884 CCATGG C^CATGG
NheI 663 GCTAGC G^CTAGC
NsiI 23 ATGCAT ATGCA^T
PflMI 3420 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1862 TCATGA T^CATGA
SacI 2074 GAGCTC GAGCT^C
SbfI 3241 CCTGCAGG CCTGCA^GG
SfiI 3370 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3349 GGCGCC GGC^GCC
SmlI 2571 CTTGAG C^T[CT][GA]AG
Sse8387I 3241 CCTGCAGG CCTGCA^GG
Van91I 3420 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 559 CCTCAGG CC^T[ACGT]AGG
1994 CCTCAGG
BanI 750 GGCACC G^G[CT][GA]CC
3349 GGCGCC
BanII 2074 GAGCTC G[GA]GC[CT]^C
2472 GAGCCC
BbsI 735 GAAGAC GAAGAC(2/6)
3258 GAAGAC
BglI 2682 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3371 GCCTCAATGGC
BpuAI 735 GAAGAC GAAGAC(2/6)
3258 GAAGAC
BsgI 1850 CTGCAC GTGCAG(16/14)
1902 GTGCAG
BsrDI 75 CATTGC GCAATG(2/0)
2325 CATTGC
Bsu36I 559 CCTCAGG CC^T[ACGT]AGG
1994 CCTCAGG
CfoI 3337 GCGC GCG^C
3350 GCGC
DraII 1643 AGGTCCC [GA]G^G[ACGT]CC[CT]
3297 GGGTCCC
DsaI 884 CCATGG C^C[GA][CT]GG
2822 CCACGG
EarI 313 GAAGAG CTCTTC(1/4)
691 GAAGAG
Eco81I 559 CCTCAGG CC^T[ACGT]AGG
1994 CCTCAGG
EcoO109I 1643 AGGTCCC [GA]G^G[ACGT]CC[CT]
3297 GGGTCCC
HaeII 3336 AGCGCC [GA]GCGC^[CT]
3349 GGCGCC
HhaI 3337 GCGC GCG^C
3350 GCGC
HinP1I 3337 GCGC G^CGC
3350 GCGC
Ksp632I 313 GAAGAG CTCTTC(1/4)
691 GAAGAG
MslI 433 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2673 CACACCCATG
PpuMI 1643 AGGTCCC [GA]G^G[AT]CC[CT]
3297 GGGTCCC
PshAI 7 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2569 GACTTGAGTC
SspI 489 AATATT AAT^ATT
3037 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
EaeI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII