Restriction map for
pFN21AE6755
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BcgI BclI BfrI BglI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI PstI PvuI
PvuII RcaI RsrII SacII SbfI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1438 GTCGAC GT^[AC][GT]AC
AclI 1332 AACGTT AA^CGTT
AcyI 69 GGCGCC G[GA]^CG[CT]C
Asp700I 496 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 581 GGTCC G^G[AT]CC
BamHI 1446 GGATCC G^GATCC
BanI 69 GGCGCC G^G[CT][GA]CC
BbeI 69 GGCGCC GGCGC^C
BbrPI 649 CACGTG CAC^GTG
BciVI 1006 GTATCC GTATCC(6/5)
BfaI 1556 CTAG C^TAG
BlpI 685 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 685 GCTCAGC GC^T[ACGT]AGC
BsaAI 649 CACGTG [CT]AC^GT[GA]
BsaHI 69 GGCGCC G[GA]^CG[CT]C
BsmAI 97 GTCTC GTCTC(1/5)
BsmI 256 GAATGC GAATGC(1/-1)
BspMI 117 ACCTGC ACCTGC(4/8)
BsrFI 1572 GCCGGC [GA]^CCGG[CT]
BstXI 472 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1446 GGATCC [GA]^GATC[CT]
CelII 685 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1572 GCCGGC [GA]^CCGG[CT]
EcoNI 1377 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 73 CCTTGG C^C[AT][AT]GG
HaeII 69 GGCGCC [GA]GCGC^[CT]
Hin1I 69 GGCGCC G[GA]^CG[CT]C
HincII 1438 GTCGAC GT[CT]^[GA]AC
HindII 1438 GTCGAC GT[CT]^[GA]AC
KasI 69 GGCGCC G^GCGCC
MaeI 1556 CTAG C^TAG
MflI 1446 GGATCC [GA]^GATC[CT]
NaeI 1572 GCCGGC GCC^GGC
NarI 69 GGCGCC GG^CGCC
NgoMIV 1572 GCCGGC G^CCGGC
NotI 27 GCGGCCGC GC^GGCCGC
PflMI 600 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 649 CACGTG CAC^GTG
PmlI 649 CACGTG CAC^GTG
PshAI 1431 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1332 AACGTT AA^CGTT
SacI 1252 GAGCTC GAGCT^C
SalI 1438 GTCGAC G^TCGAC
ScaI 1340 AGTACT AGT^ACT
SfcI 434 CTACAG C^T[GA][CT]AG
SfoI 69 GGCGCC GGC^GCC
StyI 73 CCTTGG C^C[AT][AT]GG
Van91I 600 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1555 TCTAGA T^CTAGA
XcmI 596 CCAACCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1446 GGATCC [GA]^GATC[CT]
XmnI 496 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 545 ACGTGT A^C[GA][CT]GT
648 ACACGT
AocI 1041 CCTGAGG CC^T[ACGT]AGG
1051 CCTCAGG
ApaI 670 GGGCCC GGGCC^C
1323 GGGCCC
BsgI 211 GTGCAG GTGCAG(16/14)
1645 GTGCAG
BsiEI 12 CGGCCG CG[GA][CT]^CG
28 CGGCCG
BstF5I 951 GGATG GGATG(2/0)
1527 GGATG
Bsu36I 1041 CCTGAGG CC^T[ACGT]AGG
1051 CCTCAGG
CfoI 70 GCGC GCG^C
1381 GCGC
EaeI 12 CGGCCG [CT]^GGCC[GA]
28 CGGCCG
EagI 12 CGGCCG C^GGCCG
28 CGGCCG
EclXI 12 CGGCCG C^GGCCG
28 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
28 CGGCCG
Eco81I 1041 CCTGAGG CC^T[ACGT]AGG
1051 CCTCAGG
FokI 951 GGATG GGATG(9/13)
1527 GGATG
HhaI 70 GCGC GCG^C
1381 GCGC
HinP1I 70 GCGC G^CGC
1381 GCGC
MslI 141 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
603 CACGCTGGTG
SapI 1243 GAAGAGC GCTCTTC(1/4)
1417 GAAGAGC
Tsp45I 592 GTGAC ^GT[GC]AC
1112 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvCI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I