Restriction map for
pFN21AE2517
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MspA1I MunI MvnI NarI NciI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 18 GTCGAC GT^[AC][GT]AC
AcsI 589 AAATTC [GA]^AATT[CT]
AlwNI 61 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 589 AAATTC [GA]^AATT[CT]
AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 455 GGACC G^G[AT]CC
BalI 561 TGGCCA TGG^CCA
BfaI 503 CTAG C^TAG
BlpI 66 GCTGAGC GC^T[ACGT]AGC
BmyI 700 GGGCAC G[AGT]GC[ACT]^C
BpmI 101 CTGGAG CTGGAG(16/14)
Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC
BsaAI 412 TACGTG [CT]AC^GT[GA]
BsaJI 564 CCATGG C^C[ACGT][ACGT]GG
BsaWI 395 TCCGGT [AT]^CCGG[AT]
BsmBI 104 GAGACG CGTCTC(1/5)
Bsp1286I 700 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 1201 TGTACA T^GTACA
BsrFI 27 GCCGGC [GA]^CCGG[CT]
BsrGI 1201 TGTACA T^GTACA
BstAPI 1119 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 66 GCTGAGC GC^T[ACGT]AGC
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
Cfr13I 455 GGACC G^G[ACGT]CC
DsaI 564 CCATGG C^C[GA][CT]GG
EcoT14I 564 CCATGG C^C[AT][AT]GG
FauI 31 GCGGG CCCGC(4/6)
HaeII 1304 GGCGCT [GA]GCGC^[CT]
HgaI 118 GACGC GACGC(5/10)
HincII 18 GTCGAC GT[CT]^[GA]AC
HindII 18 GTCGAC GT[CT]^[GA]AC
HphI 742 GGTGA GGTGA(8/7)
MaeI 503 CTAG C^TAG
MaeII 413 ACGT A^CGT
MluNI 561 TGGCCA TGG^CCA
MscI 561 TGGCCA TGG^CCA
NaeI 27 GCCGGC GCC^GGC
NcoI 564 CCATGG C^CATGG
NgoMIV 27 GCCGGC G^CCGGC
NspI 41 ACATGC [GA]CATG^[CT]
PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1212 GACTC GAGTC(4/5)
SalI 18 GTCGAC G^TCGAC
Sau96I 455 GGACC G^G[ACGT]CC
SgrAI 26 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 262 CTCAAG C^T[CT][GA]AG
SspBI 1201 TGTACA T^GTACA
StyI 564 CCATGG C^C[AT][AT]GG
Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 502 TCTAGA T^CTAGA
XcmI 279 CCAACCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 31 GCGG CCGC(-3/-1)
49 GCGG
BbvI 1265 GCAGC GCAGC(8/12)
1231 GCTGC
BsiYI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1295 CCAGTATTTGG
BslI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1295 CCAGTATTTGG
BsmI 813 GAATGC GAATGC(1/-1)
1268 GCATTC
BssKI 187 CCTGG ^CC[ACGT]GG
997 CCTGG
BstNI 187 CCTGG CC^[AT]GG
997 CCTGG
CfoI 25 GCGC GCG^C
1305 GCGC
EaeI 50 CGGCCA [CT]^GGCC[GA]
561 TGGCCA
EcoRII 187 CCTGG ^CC[AT]GG
997 CCTGG
HapII 28 CCGG C^CGG
396 CCGG
HhaI 25 GCGC GCG^C
1305 GCGC
HinP1I 25 GCGC G^CGC
1305 GCGC
HpaII 28 CCGG C^CGG
396 CCGG
MspI 28 CCGG C^CGG
396 CCGG
MvaI 187 CCTGG CC^[AT]GG
997 CCTGG
PflMI 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCAGTATTTGG
ScrFI 187 CCTGG CC^[ACGT]GG
997 CCTGG
SfcI 344 CTGTAG C^T[GA][CT]AG
491 CTGTAG
SspI 236 AATATT AAT^ATT
681 AATATT
TfiI 826 GAATC G^A[AT]TC
1039 GAATC
TseI 1231 GCTGC G^C[AT]GC
1265 GCAGC
Van91I 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCAGTATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AlwI BsaBI BsmAI
BsrI BstF5I BstYI Cac8I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MwoI
NdeII NlaIII RsaI Sau3AI SfaNI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XhoII