Restriction map for
pFN21AE3555
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# NO CUT ENZYMES ( 26 enzymes) #
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AscI AseI AsnI BsiWI BssHII
BstBI CpoI FseI MfeI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PmeI PshBI PvuI
RsrII SfuI SgrAI SnaBI SpeI
SwaI
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# ONE CUT ENZYMES ( 16 enzymes) #
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AclI 2446 AACGTT AA^CGTT
AgeI 661 ACCGGT A^CCGGT
BsaBI 13803 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 13705 ACATGT A^CATGT
DrdI 8761 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 8981 AAGCTT A^AGCTT
HpaI 13116 GTTAAC GTT^AAC
MamI 13803 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 10715 ACGCGT A^CGCGT
NdeI 7513 CATATG CA^TATG
PinAI 661 ACCGGT A^CCGGT
Psp1406I 2446 AACGTT AA^CGTT
SalI 7087 GTCGAC G^TCGAC
SfiI 424 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 12455 AATATT AAT^ATT
XbaI 14073 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 6881 TCCGGA T^CCGGA
10334 TCCGGA
AhdI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
12211 GACCTGGTGTC
AspEI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
12211 GACCTGGTGTC
AvrII 3112 CCTAGG C^CTAGG
11802 CCTAGG
BbeI 2077 GGCGCC GGCGC^C
6109 GGCGCC
BlnI 3112 CCTAGG C^CTAGG
11802 CCTAGG
BseAI 6881 TCCGGA T^CCGGA
10334 TCCGGA
BsiEI 7655 CGGCCG CG[GA][CT]^CG
12894 CGGCCG
BspDI 9765 ATCGAT AT^CGAT
10324 ATCGAT
BspEI 6881 TCCGGA T^CCGGA
10334 TCCGGA
Bst1107I 7693 GTATAC GTA^TAC
15144 GTATAC
BstZ17I 7693 GTATAC GTA^TAC
15144 GTATAC
ClaI 9765 ATCGAT AT^CGAT
10324 ATCGAT
EagI 7655 CGGCCG C^GGCCG
12894 CGGCCG
Eam1105I 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
12211 GACCTGGTGTC
EclXI 7655 CGGCCG C^GGCCG
12894 CGGCCG
Eco52I 7655 CGGCCG C^GGCCG
12894 CGGCCG
EcoRI 8845 GAATTC G^AATTC
12124 GAATTC
KasI 2077 GGCGCC G^GCGCC
6109 GGCGCC
MroI 6881 TCCGGA T^CCGGA
10334 TCCGGA
NarI 2077 GGCGCC GG^CGCC
6109 GGCGCC
PaeR7I 5866 CTCGAG C^TCGAG
13036 CTCGAG
SbfI 519 CCTGCAGG CCTGCA^GG
6392 CCTGCAGG
SfoI 2077 GGCGCC GGC^GCC
6109 GGCGCC
Sse8387I 519 CCTGCAGG CCTGCA^GG
6392 CCTGCAGG
XhoI 5866 CTCGAG C^TCGAG
13036 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 190 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcsI AcyI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI Asp700I
Asp718I AspHI AspI AvaI AvaII
AviII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RcaI RsaI SacI
SacII SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI