Restriction map for
pFN21AE9671
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BcgI BciVI BfrI BlnI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI BstBI ClaI CpoI
EcoRI EcoRV EcoT22I FseI FspI
HpaI KasI KpnI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfoI SfuI SpeI Sse8387I SspBI
SspI SwaI Van91I
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 314 GACGTC GACGT^C
AccIII 4085 TCCGGA T^CCGGA
AflIII 3995 ACGTGT A^C[GA][CT]GT
AhdI 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3879 GTGCAC G^TGCAC
Aor51HI 3824 AGCGCT AGC^GCT
ApaLI 3879 GTGCAC G^TGCAC
AspEI 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3994 CACGTG CAC^GTG
BseAI 4085 TCCGGA T^CCGGA
BsiEI 4392 CGGCCG CG[GA][CT]^CG
BspEI 4085 TCCGGA T^CCGGA
BspHI 1028 TCATGA T^CATGA
BsrDI 4730 GCAATG GCAATG(2/0)
Bst1107I 4368 GTATAC GTA^TAC
BstEII 1249 GGTCACC G^GT[ACGT]ACC
BstPI 1249 GGTCACC G^GT[ACGT]ACC
BstZ17I 4368 GTATAC GTA^TAC
DraI 255 TTTAAA TTT^AAA
DrdI 731 GACTCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4392 CGGCCG C^GGCCG
Eam1105I 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4392 CGGCCG C^GGCCG
Eco47III 3824 AGCGCT AGC^GCT
Eco52I 4392 CGGCCG C^GGCCG
EcoO65I 1249 GGTCACC G^GT[ACGT]ACC
MroI 4085 TCCGGA T^CCGGA
PmaCI 3994 CACGTG CAC^GTG
PmlI 3994 CACGTG CAC^GTG
RcaI 1028 TCATGA T^CATGA
SacI 4071 GAGCTC GAGCT^C
SexAI 4063 ACCAGGT A^CC[AT]GGT
SfiI 39 GGCCGTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1948 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 203 TACGTA TAC^GTA
XbaI 318 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1870 GTCTAC GT^[AC][GT]AC
4368 GTATAC
ApaI 519 GGGCCC GGGCC^C
4409 GGGCCC
BamHI 1467 GGATCC G^GATCC
2547 GGATCC
BbvCI 3753 CCTCAGC CCTCAGC(-5/-2)
4811 CCTCAGC
BglI 40 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2689 GCCCGTCTGGC
BlpI 439 GCTAAGC GC^T[ACGT]AGC
4020 GCTGAGC
Bpu1102I 439 GCTAAGC GC^T[ACGT]AGC
4020 GCTGAGC
BsaAI 203 TACGTA [CT]AC^GT[GA]
3994 CACGTG
BsaBI 1515 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATAGAGATC
BsaI 1594 GAGACC GGTCTC(1/5)
3604 GAGACC
BsaWI 2918 ACCGGA [AT]^CCGG[AT]
4085 TCCGGA
BsmI 1058 GCATTC GAATGC(1/-1)
1815 GCATTC
BsrBI 4662 CCGCTC CCGCTC(-3/-3)
2062 GAGCGG
BstAPI 1058 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4445 GCAAAGCCTGC
CelII 439 GCTAAGC GC^T[ACGT]AGC
4020 GCTGAGC
DraIII 3486 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4633 CACCGTGTG
EcoNI 2559 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4119 CCTGCCGGAGG
HincII 863 GTCAAC GT[CT]^[GA]AC
3052 GTCAAC
HindII 863 GTCAAC GT[CT]^[GA]AC
3052 GTCAAC
HindIII 3282 AAGCTT A^AGCTT
4777 AAGCTT
KspI 3618 CCGCGG CCGC^GG
3950 CCGCGG
MamI 1515 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATAGAGATC
PvuII 2596 CAGCTG CAG^CTG
4018 CAGCTG
SacII 3618 CCGCGG CCGC^GG
3950 CCGCGG
SapI 3009 GAAGAGC GCTCTTC(1/4)
4692 GCTCTTC
SmaI 23 CCCGGG CCC^GGG
4007 CCCGGG
SphI 1739 GCATGC GCATG^C
4271 GCATGC
XcmI 1856 CCACCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4294 CCAGGGTCAGACTGG
XmaCI 23 CCCGGG C^CCGGG
4007 CCCGGG
XmaI 23 CCCGGG C^CCGGG
4007 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PflFI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoI XhoII XmnI