Restriction map for
pFN21AB8030
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 1380 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1473 GGATCC G^GATCC
BbvCI 146 CCTCAGC CCTCAGC(-5/-2)
BcgI 1611 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1218 GGATAC GTATCC(6/5)
BglI 919 GCCAATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 3 GTGCCC G[AGT]GC[ACT]^C
BpmI 1634 CTGGAG CTGGAG(16/14)
BsaBI 1357 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1408 CTCCTC GAGGAG(10/8)
BsgI 616 CTGCAC GTGCAG(16/14)
BsmI 1560 GCATTC GAATGC(1/-1)
Bsp1286I 3 GTGCCC G[AGT]GC[ACT]^C
BsrBI 1405 CCGCTC CCGCTC(-3/-3)
BsrDI 1299 CATTGC GCAATG(2/0)
BstAPI 1045 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1341 GGTGACC G^GT[ACGT]ACC
BstPI 1341 GGTGACC G^GT[ACGT]ACC
BstYI 1473 GGATCC [GA]^GATC[CT]
Cac8I 638 GCAAGC GC[ACGT]^[ACGT]GC
DraIII 1177 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 290 CCTACGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1341 GGTGACC G^GT[ACGT]ACC
EcoT14I 116 CCAAGG C^C[AT][AT]GG
MaeII 958 ACGT A^CGT
MamI 1357 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1473 GGATCC [GA]^GATC[CT]
PflFI 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 1453 ACCAGGT A^CC[AT]GGT
SspI 753 AATATT AAT^ATT
StyI 116 CCAAGG C^C[AT][AT]GG
SwaI 698 ATTTAAAT ATTT^AAAT
TaqI 1034 TCGA T^CGA
Tth111I 1489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1034 TCGA T^CGA
XhoII 1473 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 1348 ACATGT A^C[GA][CT]GT
1659 ACATGT
BfaI 674 CTAG C^TAG
785 CTAG
BspLU11I 1348 ACATGT A^CATGT
1659 ACATGT
DpnII 478 GATC ^GATC
1474 GATC
DraI 604 TTTAAA TTT^AAA
699 TTTAAA
EcoRI 27 GAATTC G^AATTC
1248 GAATTC
FauI 916 CCCGC CCCGC(4/6)
1649 GCGGG
HapII 925 CCGG C^CGG
1208 CCGG
HpaII 925 CCGG C^CGG
1208 CCGG
MaeI 674 CTAG C^TAG
785 CTAG
MboI 478 GATC ^GATC
1474 GATC
MslI 1075 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1225 CATATTTATG
MspI 925 CCGG C^CGG
1208 CCGG
NdeII 478 GATC ^GATC
1474 GATC
NspI 1348 ACATGT [GA]CATG^[CT]
1659 ACATGT
PleI 371 GACTC GAGTC(4/5)
358 GAGTC
PvuII 149 CAGCTG CAG^CTG
1383 CAGCTG
Sau3AI 478 GATC ^GATC
1474 GATC
XcmI 257 CCACACCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1139 CCAGTTCTTCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BpuAI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI