Restriction map for
pFN21AE2378
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfrI
BglI BglII BlnI BmyI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 6 GTAC GT^AC
AflIII 251 ACATGT A^C[GA][CT]GT
AlwI 1201 GATCC GGATC(4/5)
Asp700I 610 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1015 GGACC G^G[AT]CC
AviII 1112 TGCGCA TGC^GCA
BbsI 1174 GAAGAC GAAGAC(2/6)
BclI 1308 TGATCA T^GATCA
BlpI 1452 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1452 GCTCAGC GC^T[ACGT]AGC
BpuAI 1174 GAAGAC GAAGAC(2/6)
BsaBI 529 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1400 CCAAGG C^C[ACGT][ACGT]GG
BseRI 899 GAGGAG GAGGAG(10/8)
BsgI 788 GTGCAG GTGCAG(16/14)
BsiYI 619 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 619 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 1170 TCATGA T^CATGA
BspLU11I 251 ACATGT A^CATGT
BstXI 597 CCATAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1200 AGATCC [GA]^GATC[CT]
CelII 1452 GCTCAGC GC^T[ACGT]AGC
CfoI 1113 GCGC GCG^C
DraII 166 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1130 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 619 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 166 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1258 GAATTC G^AATTC
EcoT14I 1400 CCAAGG C^C[AT][AT]GG
FbaI 1308 TGATCA T^GATCA
Fnu4HI 20 GCGGC GC^[ACGT]GC
FspI 1112 TGCGCA TGC^GCA
HaeIII 167 GGCC GG^CC
HhaI 1113 GCGC GCG^C
HinP1I 1113 GCGC G^CGC
HindIII 557 AAGCTT A^AGCTT
ItaI 20 GCGGC GC^[ACGT]GC
MamI 529 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1200 AGATCC [GA]^GATC[CT]
MwoI 1324 GCAAAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 950 CTGCAG CTGCA^G
RcaI 1170 TCATGA T^CATGA
RsaI 6 GTAC GT^AC
SexAI 1131 ACCTGGT A^CC[AT]GGT
SfcI 950 CTGCAG C^T[GA][CT]AG
SmlI 633 CTTGAG C^T[CT][GA]AG
StyI 1400 CCAAGG C^C[AT][AT]GG
Tth111I 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1200 AGATCC [GA]^GATC[CT]
XmnI 610 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 353 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1265 CAGATTCTG
BfaI 764 CTAG C^TAG
1334 CTAG
BsmAI 629 GAGAC GTCTC(1/5)
946 GAGAC
BssKI 619 CCTGG ^CC[ACGT]GG
1132 CCTGG
BstNI 619 CCTGG CC^[AT]GG
1132 CCTGG
Cac8I 474 GCTTGC GC[ACGT]^[ACGT]GC
1146 GCAAGC
Cfr13I 167 GGCCC G^G[ACGT]CC
1015 GGACC
EcoRII 619 CCTGG ^CC[AT]GG
1132 CCTGG
MaeI 764 CTAG C^TAG
1334 CTAG
MaeII 458 ACGT A^CGT
1379 ACGT
MaeIII 455 GTTAC ^GT[ACGT]AC
1119 GTTAC
MvaI 619 CCTGG CC^[AT]GG
1132 CCTGG
NdeI 710 CATATG CA^TATG
941 CATATG
NlaIV 167 GGCCCC GG[ACGT]^[ACGT]CC
1435 GGAACC
NspI 251 ACATGT [GA]CATG^[CT]
1115 GCATGT
Sau96I 167 GGCCC G^G[ACGT]CC
1015 GGACC
ScrFI 619 CCTGG CC^[ACGT]GG
1132 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AcsI AluI ApoI BpmI
BsrI BstF5I DdeI DpnII FokI
HinfI HphI MboI MboII MnlI
MseI MslI NdeII NlaIII PleI
Sau3AI SfaNI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I