Restriction map for
pFN21AE2367
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# NO CUT ENZYMES ( 57 enzymes) #
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Acc65I AccIII AgeI Aor51HI AscI
Asp718I AspI AvrII BglII BlnI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI DrdI
Eco47III EcoRI FseI KpnI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI PacI PflFI
PinAI PmeI PshAI PvuI RsrII
SalI ScaI SgrAI SmaI SnaBI
SpeI SphI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 70 GTCTAC GT^[AC][GT]AC
AclI 4734 AACGTT AA^CGTT
AflII 4423 CTTAAG C^TTAAG
Alw44I 1061 GTGCAC G^TGCAC
ApaLI 1061 GTGCAC G^TGCAC
AseI 2076 ATTAAT AT^TAAT
AsnI 2076 ATTAAT AT^TAAT
Asp700I 1418 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5748 TGCGCA TGC^GCA
BalI 596 TGGCCA TGG^CCA
BanI 5587 GGCGCC G^G[CT][GA]CC
BbeI 5587 GGCGCC GGCGC^C
BbrPI 3184 CACGTG CAC^GTG
BbsI 1831 GTCTTC GAAGAC(2/6)
BcgI 5617 GCAATCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5781 GTATCC GTATCC(6/5)
BfrI 4423 CTTAAG C^TTAAG
BpuAI 1831 GTCTTC GAAGAC(2/6)
BsaAI 3184 CACGTG [CT]AC^GT[GA]
Bsp1407I 3802 TGTACA T^GTACA
BsrGI 3802 TGTACA T^GTACA
BstBI 2907 TTCGAA TT^CGAA
BstEII 1907 GGTCACC G^GT[ACGT]ACC
BstPI 1907 GGTCACC G^GT[ACGT]ACC
BstXI 1574 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3594 TTTAAA TTT^AAA
EcoNI 6129 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1907 GGTCACC G^GT[ACGT]ACC
FspI 5748 TGCGCA TGC^GCA
HpaI 4477 GTTAAC GTT^AAC
KasI 5587 GGCGCC G^GCGCC
KspI 1903 CCGCGG CCGC^GG
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
NarI 5587 GGCGCC GG^CGCC
NheI 705 GCTAGC G^CTAGC
NspV 2907 TTCGAA TT^CGAA
PflMI 5426 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3184 CACGTG CAC^GTG
PmlI 3184 CACGTG CAC^GTG
PshBI 2076 ATTAAT AT^TAAT
Psp1406I 4734 AACGTT AA^CGTT
SacI 3410 GAGCTC GAGCT^C
SacII 1903 CCGCGG CCGC^GG
SbfI 5521 CCTGCAGG CCTGCA^GG
SexAI 1793 ACCTGGT A^CC[AT]GGT
SfiI 1003 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5587 GGCGCC GGC^GCC
SfuI 2907 TTCGAA TT^CGAA
Sse8387I 5521 CCTGCAGG CCTGCA^GG
SspBI 3802 TGTACA T^GTACA
Van91I 5426 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1418 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AatII 67 GACGTC GACGT^C
3301 GACGTC
AccII 1904 CGCG CG^CG
4532 CGCG
AocI 5194 CCTGAGG CC^T[ACGT]AGG
6133 CCTCAGG
ApaI 989 GGGCCC GGGCC^C
4628 GGGCCC
BbvCI 2957 CCTCAGC CCTCAGC(-5/-2)
5767 CCTCAGC
BglI 1004 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5911 GCCTATCCGGC
BsmI 2001 GAATGC GAATGC(1/-1)
3639 GAATGC
BssSI 4179 CACGAG CACGAG(-5/-1)
5446 CTCGTG
BstAPI 4536 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6312 GCAAGGCTTGC
BstUI 1904 CGCG CG^CG
4532 CGCG
Bsu36I 5194 CCTGAGG CC^T[ACGT]AGG
6133 CCTCAGG
DraIII 4608 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5427 CACCTTGTG
Eco81I 5194 CCTGAGG CC^T[ACGT]AGG
6133 CCTCAGG
EcoT22I 2778 ATGCAT ATGCA^T
4757 ATGCAT
MvnI 1904 CGCG CG^CG
4532 CGCG
NcoI 1436 CCATGG C^CATGG
1574 CCATGG
NsiI 2778 ATGCAT ATGCA^T
4757 ATGCAT
PaeR7I 978 CTCGAG C^TCGAG
1868 CTCGAG
PpuMI 80 AGGACCC [GA]G^G[AT]CC[CT]
4016 AGGACCC
PvuII 1033 CAGCTG CAG^CTG
2227 CAGCTG
XhoI 978 CTCGAG C^TCGAG
1868 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaI AvaII BamHI
BanII BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoO109I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII