Restriction map for
pFN21AE9275
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
Acc65I AccI AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 396 GACGTC GACGT^C
AcsI 286 GAATTT [GA]^AATT[CT]
AcyI 396 GACGTC G[GA]^CG[CT]C
AlwNI 587 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 45 CCTGAGG CC^T[ACGT]AGG
ApoI 286 GAATTT [GA]^AATT[CT]
AseI 268 ATTAAT AT^TAAT
AsnI 268 ATTAAT AT^TAAT
AvaI 52 CTCGGG C^[CT]CG[GA]G
BamHI 199 GGATCC G^GATCC
BcgI 375 GCAGCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 396 GACGTC G[GA]^CG[CT]C
BseRI 659 CTCCTC GAGGAG(10/8)
BsiEI 73 CGGCCG CG[GA][CT]^CG
BsmFI 7 GGGAC GGGAC(10/14)
BsmI 314 GCATTC GAATGC(1/-1)
BsoBI 52 CTCGGG C^[CT]CG[GA]G
BsrDI 40 CATTGC GCAATG(2/0)
BsrFI 722 GCCGGC [GA]^CCGG[CT]
BstF5I 510 GGATG GGATG(2/0)
BstYI 199 GGATCC [GA]^GATC[CT]
Bsu36I 45 CCTGAGG CC^T[ACGT]AGG
Cfr10I 722 GCCGGC [GA]^CCGG[CT]
DpnII 200 GATC ^GATC
DraI 109 TTTAAA TTT^AAA
DsaI 76 CCGCGG C^C[GA][CT]GG
EagI 73 CGGCCG C^GGCCG
EclXI 73 CGGCCG C^GGCCG
Eco52I 73 CGGCCG C^GGCCG
Eco81I 45 CCTGAGG CC^T[ACGT]AGG
FokI 510 GGATG GGATG(9/13)
HapII 723 CCGG C^CGG
Hin1I 396 GACGTC G[GA]^CG[CT]C
HincII 541 GTTGAC GT[CT]^[GA]AC
HindII 541 GTTGAC GT[CT]^[GA]AC
HpaII 723 CCGG C^CGG
KspI 76 CCGCGG CCGC^GG
MaeII 397 ACGT A^CGT
MboI 200 GATC ^GATC
MflI 199 GGATCC [GA]^GATC[CT]
MspI 723 CCGG C^CGG
NaeI 722 GCCGGC GCC^GGC
NdeII 200 GATC ^GATC
NgoMIV 722 GCCGGC G^CCGGC
NotI 72 GCGGCCGC GC^GGCCGC
PshBI 268 ATTAAT AT^TAAT
SacII 76 CCGCGG CCGC^GG
Sau3AI 200 GATC ^GATC
SfaNI 118 GCATC GCATC(5/9)
SfcI 122 CTATAG C^T[GA][CT]AG
StuI 697 AGGCCT AGG^CCT
TaqI 65 TCGA T^CGA
TthHB8I 65 TCGA T^CGA
XhoII 199 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 31 CGCG CG^CG
77 CGCG
AlwI 200 GATCC GGATC(4/5)
199 GGATC
AspHI 437 GAGCTC G[AT]GC[AT]^C
597 GAGCTC
BanII 437 GAGCTC G[GA]GC[CT]^C
597 GAGCTC
BbvCI 337 GCTGAGG CCTCAGC(-5/-2)
528 GCTGAGG
BmyI 437 GAGCTC G[AGT]GC[ACT]^C
597 GAGCTC
BpmI 452 CTCCAG CTGGAG(16/14)
624 CTCCAG
BsaJI 76 CCGCGG C^C[ACGT][ACGT]GG
195 CCCTGG
BsiHKAI 437 GAGCTC G[AT]GC[AT]^C
597 GAGCTC
BsiYI 18 CCCGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCTCCAGTTGG
BslI 18 CCCGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCTCCAGTTGG
Bsp1286I 437 GAGCTC G[AGT]GC[ACT]^C
597 GAGCTC
BspHI 273 TCATGA T^CATGA
746 TCATGA
BsrI 280 ACTGG ACTGG(1/-1)
454 CCAGT
BssKI 196 CCTGG ^CC[ACGT]GG
357 CCAGG
BstNI 196 CCTGG CC^[AT]GG
357 CCAGG
BstUI 31 CGCG CG^CG
77 CGCG
EaeI 73 CGGCCG [CT]^GGCC[GA]
724 CGGCCA
EarI 582 CTCTTC CTCTTC(1/4)
600 CTCTTC
EcoRII 196 CCTGG ^CC[AT]GG
357 CCAGG
FauI 18 CCCGC CCCGC(4/6)
78 GCGGG
HinfI 589 GACTC G^A[ACGT]TC
781 GAGTC
Ksp632I 582 CTCTTC CTCTTC(1/4)
600 CTCTTC
MseI 110 TTAA T^TAA
269 TTAA
MslI 213 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
315 CATTCAGGTG
MspA1I 19 CCGCTG C[AC]G^C[GT]G
76 CCGCGG
MvaI 196 CCTGG CC^[AT]GG
357 CCAGG
MvnI 31 CGCG CG^CG
77 CGCG
MwoI 569 GCAGTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
629 GCAGTGGAGGC
PleI 589 GACTC GAGTC(4/5)
781 GAGTC
RcaI 273 TCATGA T^CATGA
746 TCATGA
SacI 437 GAGCTC GAGCT^C
597 GAGCTC
SapI 581 GCTCTTC GCTCTTC(1/4)
599 GCTCTTC
ScrFI 196 CCTGG CC^[ACGT]GG
357 CCAGG
Tru9I 110 TTAA T^TAA
269 TTAA
Tsp509I 177 AATT ^AATT
287 AATT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI AvaII BbvI Cac8I
Cfr13I DdeI Fnu4HI HaeIII HphI
ItaI MaeIII MboII MnlI NlaIII
NlaIV Sau96I TseI Tsp45I TspRI