Restriction map for
pFN21AB3035
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AlwI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsiWI
BsmI BspDI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MflI MluI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 866 TCCGGA T^CCGGA
AflIII 772 ACGTGT A^C[GA][CT]GT
Alw44I 1418 GTGCAC G^TGCAC
AocI 102 CCTGAGG CC^T[ACGT]AGG
ApaLI 1418 GTGCAC G^TGCAC
Asp700I 1578 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 837 GGACC G^G[AT]CC
BalI 746 TGGCCA TGG^CCA
BfaI 1000 CTAG C^TAG
BsaI 908 GAGACC GGTCTC(1/5)
BsaWI 866 TCCGGA [AT]^CCGG[AT]
BseAI 866 TCCGGA T^CCGGA
BspEI 866 TCCGGA T^CCGGA
BsrDI 1202 GCAATG GCAATG(2/0)
BstAPI 1716 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 542 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 102 CCTGAGG CC^T[ACGT]AGG
DpnII 351 GATC ^GATC
DraIII 354 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1156 CCGCGG C^C[GA][CT]GG
Eco81I 102 CCTGAGG CC^T[ACGT]AGG
EcoRV 1020 GATATC GAT^ATC
HincII 1054 GTCAAC GT[CT]^[GA]AC
HindII 1054 GTCAAC GT[CT]^[GA]AC
KspI 1156 CCGCGG CCGC^GG
MaeI 1000 CTAG C^TAG
MaeIII 623 GTGAC ^GT[ACGT]AC
MboI 351 GATC ^GATC
MluNI 746 TGGCCA TGG^CCA
MroI 866 TCCGGA T^CCGGA
MscI 746 TGGCCA TGG^CCA
NdeII 351 GATC ^GATC
NspI 791 GCATGT [GA]CATG^[CT]
PaeR7I 1709 CTCGAG C^TCGAG
PflMI 738 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 373 GACTC GAGTC(4/5)
SacI 1040 GAGCTC GAGCT^C
SacII 1156 CCGCGG CCGC^GG
Sau3AI 351 GATC ^GATC
SfiI 1213 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1709 CTCGAG C^T[CT][GA]AG
StuI 1088 AGGCCT AGG^CCT
TfiI 136 GATTC G^A[AT]TC
Tsp45I 623 GTGAC ^GT[GC]AC
Van91I 738 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1709 CTCGAG C^TCGAG
XmnI 1578 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AcyI 1515 GACGCC G[GA]^CG[CT]C
1660 GACGCC
AlwNI 841 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
844 CAGCTGCTG
BanI 516 GGTGCC G^G[CT][GA]CC
574 GGTGCC
BbrPI 79 CACGTG CAC^GTG
771 CACGTG
BbvCI 1131 CCTCAGC CCTCAGC(-5/-2)
1384 GCTGAGG
BcgI 1581 CGACTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1725 GCAGGAATATCG
BlpI 667 GCTGAGC GC^T[ACGT]AGC
1042 GCTCAGC
Bpu1102I 667 GCTGAGC GC^T[ACGT]AGC
1042 GCTCAGC
BsaAI 79 CACGTG [CT]AC^GT[GA]
771 CACGTG
BsaHI 1515 GACGCC G[GA]^CG[CT]C
1660 GACGCC
BsiEI 252 CGGCCG CG[GA][CT]^CG
1221 CGGCCG
BsmBI 418 GAGACG CGTCTC(1/5)
1513 GAGACG
BsmFI 836 GGGAC GGGAC(10/14)
606 GTCCC
Bsp1407I 775 TGTACA T^GTACA
794 TGTACA
BsrGI 775 TGTACA T^GTACA
794 TGTACA
CelII 667 GCTGAGC GC^T[ACGT]AGC
1042 GCTCAGC
EagI 252 CGGCCG C^GGCCG
1221 CGGCCG
EarI 1551 CTCTTC CTCTTC(1/4)
567 GAAGAG
EclXI 252 CGGCCG C^GGCCG
1221 CGGCCG
Eco52I 252 CGGCCG C^GGCCG
1221 CGGCCG
EcoNI 699 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
735 CCTCCAGCAGG
EcoT14I 177 CCAAGG C^C[AT][AT]GG
903 CCTTGG
Hin1I 1515 GACGCC G[GA]^CG[CT]C
1660 GACGCC
HinfI 136 GATTC G^A[ACGT]TC
373 GACTC
Ksp632I 1551 CTCTTC CTCTTC(1/4)
567 GAAGAG
PmaCI 79 CACGTG CAC^GTG
771 CACGTG
PmlI 79 CACGTG CAC^GTG
771 CACGTG
PvuII 844 CAGCTG CAG^CTG
1846 CAGCTG
SfaNI 284 GCATC GCATC(5/9)
1634 GCATC
SmaI 1104 CCCGGG CCC^GGG
1397 CCCGGG
SspBI 775 TGTACA T^GTACA
794 TGTACA
StyI 177 CCAAGG C^C[AT][AT]GG
903 CCTTGG
XmaCI 1104 CCCGGG C^CCGGG
1397 CCCGGG
XmaI 1104 CCCGGG C^CCGGG
1397 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AspHI AvaI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfcI TaqI
TseI TspRI TthHB8I