Restriction map for
pFN21AE2331
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AviII AvrII BamHI BbrPI BbvCI
BcgI BfrI BlnI BsaAI BsgI
BsiEI BsiWI BsmBI BspDI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HpaI KpnI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 1187 TCCGGA T^CCGGA
AcyI 46 GGCGCC G[GA]^CG[CT]C
AflIII 831 ACATGT A^C[GA][CT]GT
AhdI 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1783 CCTCAGG CC^T[ACGT]AGG
AspEI 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 46 GGCGCC GGCGC^C
BglI 844 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1277 AGATCT A^GATCT
BlpI 2067 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2067 GCTCAGC GC^T[ACGT]AGC
BsaBI 666 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
BsaWI 1187 TCCGGA [AT]^CCGG[AT]
BseAI 1187 TCCGGA T^CCGGA
BsmI 2007 GAATGC GAATGC(1/-1)
Bsp1407I 1052 TGTACA T^GTACA
BspEI 1187 TCCGGA T^CCGGA
BspHI 437 TCATGA T^CATGA
BspLU11I 831 ACATGT A^CATGT
BsrBI 402 GAGCGG CCGCTC(-3/-3)
BsrDI 197 GCAATG GCAATG(2/0)
BsrFI 1824 GCCGGT [GA]^CCGG[CT]
BsrGI 1052 TGTACA T^GTACA
BstBI 980 TTCGAA TT^CGAA
BstXI 56 CCAAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1783 CCTCAGG CC^T[ACGT]AGG
CelII 2067 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1824 GCCGGT [GA]^CCGG[CT]
DraII 1675 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1774 GAAGAG CTCTTC(1/4)
Eco81I 1783 CCTCAGG CC^T[ACGT]AGG
EcoNI 1679 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1675 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1834 GATATC GAT^ATC
HaeII 46 GGCGCC [GA]GCGC^[CT]
Hin1I 46 GGCGCC G[GA]^CG[CT]C
HincII 1093 GTCAAC GT[CT]^[GA]AC
HindII 1093 GTCAAC GT[CT]^[GA]AC
HindIII 1072 AAGCTT A^AGCTT
KasI 46 GGCGCC G^GCGCC
Ksp632I 1774 GAAGAG CTCTTC(1/4)
KspI 11 CCGCGG CCGC^GG
MaeII 343 ACGT A^CGT
MamI 666 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1187 TCCGGA T^CCGGA
NarI 46 GGCGCC GG^CGCC
NdeI 1870 CATATG CA^TATG
NspV 980 TTCGAA TT^CGAA
PaeR7I 282 CTCGAG C^TCGAG
PpuMI 1675 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 877 CAGCTG CAG^CTG
RcaI 437 TCATGA T^CATGA
SacII 11 CCGCGG CCGC^GG
ScaI 1982 AGTACT AGT^ACT
SfoI 46 GGCGCC GGC^GCC
SfuI 980 TTCGAA TT^CGAA
SmaI 332 CCCGGG CCC^GGG
SphI 156 GCATGC GCATG^C
SspBI 1052 TGTACA T^GTACA
XhoI 282 CTCGAG C^TCGAG
XmaCI 332 CCCGGG C^CCGGG
XmaI 332 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 1381 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1519 CAGAACCTG
AseI 739 ATTAAT AT^TAAT
1084 ATTAAT
AsnI 739 ATTAAT AT^TAAT
1084 ATTAAT
AspI 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACTTCGTC
BanI 46 GGCGCC G^G[CT][GA]CC
1885 GGTGCC
BanII 842 GAGCCC G[GA]GC[CT]^C
2146 GAGCCC
BciVI 519 GGATAC GTATCC(6/5)
1376 GTATCC
BclI 72 TGATCA T^GATCA
1640 TGATCA
BfaI 1301 CTAG C^TAG
1487 CTAG
BpmI 151 CTCCAG CTGGAG(16/14)
239 CTCCAG
BsaI 1306 GAGACC GGTCTC(1/5)
122 GGTCTC
FbaI 72 TGATCA T^GATCA
1640 TGATCA
HgaI 2043 GACGC GACGC(5/10)
2122 GACGC
MaeI 1301 CTAG C^TAG
1487 CTAG
MslI 387 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1171 CACCAAGATG
NcoI 501 CCATGG C^CATGG
1103 CCATGG
PflFI 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACTTCGTC
PflMI 32 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
648 CCAGAAAGTGG
PshBI 739 ATTAAT AT^TAAT
1084 ATTAAT
PstI 592 CTGCAG CTGCA^G
1516 CTGCAG
SfcI 592 CTGCAG C^T[GA][CT]AG
1516 CTGCAG
SmlI 282 CTCGAG C^T[CT][GA]AG
1198 CTTGAG
SspI 140 AATATT AAT^ATT
790 AATATT
Tsp45I 340 GTCAC ^GT[GC]AC
1849 GTGAC
Tth111I 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACTTCGTC
Van91I 32 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
648 CCAGAAAGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BbsI
BbvI BcnI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII