Restriction map for
pFN21AE1254
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AfaI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
AvrII BanI BbeI BbrPI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PstI PvuI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 1073 AACGTT AA^CGTT
AhdI 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 439 ATTAAT AT^TAAT
AsnI 439 ATTAAT AT^TAAT
AspEI 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 851 GGATCC G^GATCC
BanII 869 GAGCTC G[GA]GC[CT]^C
BbvCI 984 GCTGAGG CCTCAGC(-5/-2)
BcgI 756 CGACGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1405 GCCCAACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 874 CTCCAG CTGGAG(16/14)
BsgI 764 GTGCAG GTGCAG(16/14)
BsmAI 1245 GTCTC GTCTC(1/5)
Bst1107I 348 GTATAC GTA^TAC
BstYI 851 GGATCC [GA]^GATC[CT]
BstZ17I 348 GTATAC GTA^TAC
Cac8I 750 GCATGC GC[ACGT]^[ACGT]GC
DraI 496 TTTAAA TTT^AAA
DraII 1113 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1113 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 770 CCATGG C^C[AT][AT]GG
MfeI 419 CAATTG C^AATTG
MflI 851 GGATCC [GA]^GATC[CT]
MspA1I 560 CAGCTG C[AC]G^C[GT]G
MunI 419 CAATTG C^AATTG
NcoI 770 CCATGG C^CATGG
NspI 750 GCATGC [GA]CATG^[CT]
PflMI 1130 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1113 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1128 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 439 ATTAAT AT^TAAT
Psp1406I 1073 AACGTT AA^CGTT
PvuII 560 CAGCTG CAG^CTG
SacI 869 GAGCTC GAGCT^C
SfaNI 786 GATGC GCATC(5/9)
SfcI 587 CTACAG C^T[GA][CT]AG
SmlI 706 CTCAAG C^T[CT][GA]AG
SphI 750 GCATGC GCATG^C
StyI 770 CCATGG C^C[AT][AT]GG
Tsp45I 401 GTGAC ^GT[GC]AC
Van91I 1130 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 851 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 3 GTCTAC GT^[AC][GT]AC
348 GTATAC
AcsI 519 AAATTT [GA]^AATT[CT]
1491 AAATTC
AlwI 852 GATCC GGATC(4/5)
851 GGATC
ApoI 519 AAATTT [GA]^AATT[CT]
1491 AAATTC
Asp700I 726 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1579 GAAGCAATTC
BciVI 994 GGATAC GTATCC(6/5)
1102 GGATAC
BfaI 97 CTAG C^TAG
1154 CTAG
BspHI 863 TCATGA T^CATGA
1563 TCATGA
BstF5I 1196 CATCC GGATG(2/0)
54 GGATG
DpnII 852 GATC ^GATC
1556 GATC
DsaI 140 CCGTGG C^C[GA][CT]GG
770 CCATGG
FauI 689 CCCGC CCCGC(4/6)
1614 GCGGG
FokI 1196 CATCC GGATG(9/13)
54 GGATG
HgaI 601 GACGC GACGC(5/10)
978 GACGC
MaeI 97 CTAG C^TAG
1154 CTAG
MaeIII 323 GTAAC ^GT[ACGT]AC
401 GTGAC
MboI 852 GATC ^GATC
1556 GATC
MslI 571 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
717 CACTGTGGTG
NdeII 852 GATC ^GATC
1556 GATC
RcaI 863 TCATGA T^CATGA
1563 TCATGA
SapI 301 GAAGAGC GCTCTTC(1/4)
1157 GCTCTTC
Sau3AI 852 GATC ^GATC
1556 GATC
TaqI 47 TCGA T^CGA
824 TCGA
TfiI 40 GAATC G^A[AT]TC
1569 GAATC
TthHB8I 47 TCGA T^CGA
824 TCGA
XmnI 726 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1579 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BbsI BbvI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrDI BssKI BstNI Cfr13I
DdeI EaeI EarI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MboII MluNI MnlI MscI
MseI MvaI MwoI NlaIII NlaIV
PleI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI