Restriction map for
pFN21AE3432
##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI KpnI KspI MaeII
MamI MfeI MluI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 923 TCCGGA T^CCGGA
AflIII 1157 ACATGT A^C[GA][CT]GT
AspI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1319 GGCGCC GGCGC^C
BbvCI 2326 CCTCAGC CCTCAGC(-5/-2)
BcgI 390 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1864 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 923 TCCGGA [AT]^CCGG[AT]
BseAI 923 TCCGGA T^CCGGA
BsgI 1530 CTGCAC GTGCAG(16/14)
BsmI 2136 GAATGC GAATGC(1/-1)
BspEI 923 TCCGGA T^CCGGA
BspLU11I 1157 ACATGT A^CATGT
BspMI 692 ACCTGC ACCTGC(4/8)
EcoRI 731 GAATTC G^AATTC
HincII 985 GTTAAC GT[CT]^[GA]AC
HindII 985 GTTAAC GT[CT]^[GA]AC
HindIII 2000 AAGCTT A^AGCTT
HpaI 985 GTTAAC GTT^AAC
KasI 1319 GGCGCC G^GCGCC
MroI 923 TCCGGA T^CCGGA
NarI 1319 GGCGCC GG^CGCC
PaeR7I 290 CTCGAG C^TCGAG
PflFI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 266 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1356 AGTACT AGT^ACT
SexAI 1520 ACCTGGT A^CC[AT]GGT
SfoI 1319 GGCGCC GGC^GCC
SmaI 500 CCCGGG CCC^GGG
SpeI 1023 ACTAGT A^CTAGT
StuI 1919 AGGCCT AGG^CCT
Tth111I 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 290 CTCGAG C^TCGAG
XmaCI 500 CCCGGG C^CCGGG
XmaI 500 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1570 GTAGAC GT^[AC][GT]AC
1807 GTAGAC
AspHI 137 GTGCTC G[AT]GC[AT]^C
1448 GTGCTC
BalI 809 TGGCCA TGG^CCA
1626 TGGCCA
BbsI 340 GAAGAC GAAGAC(2/6)
1413 GAAGAC
BlpI 751 GCTCAGC GC^T[ACGT]AGC
1243 GCTGAGC
Bpu1102I 751 GCTCAGC GC^T[ACGT]AGC
1243 GCTGAGC
BpuAI 340 GAAGAC GAAGAC(2/6)
1413 GAAGAC
BsiHKAI 137 GTGCTC G[AT]GC[AT]^C
1448 GTGCTC
BsrBI 1144 GAGCGG CCGCTC(-3/-3)
1246 GAGCGG
BsrDI 215 CATTGC GCAATG(2/0)
1728 CATTGC
BsrFI 791 ACCGGC [GA]^CCGG[CT]
2169 ACCGGC
CelII 751 GCTCAGC GC^T[ACGT]AGC
1243 GCTGAGC
Cfr10I 791 ACCGGC [GA]^CCGG[CT]
2169 ACCGGC
DrdI 189 GACAAATAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
570 GACCCCTGAGTC
DsaI 369 CCACGG C^C[GA][CT]GG
1610 CCACGG
EaeI 809 TGGCCA [CT]^GGCC[GA]
1626 TGGCCA
MluNI 809 TGGCCA TGG^CCA
1626 TGGCCA
MscI 809 TGGCCA TGG^CCA
1626 TGGCCA
MseI 281 TTAA T^TAA
986 TTAA
MslI 1212 CACGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1309 CACTATGATG
PpuMI 264 GGGACCC [GA]G^G[AT]CC[CT]
374 GGGACCT
TfiI 89 GATTC G^A[AT]TC
1036 GATTC
Tru9I 281 TTAA T^TAA
986 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AvaI
AvaII BamHI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII