Restriction map for
pFN21AE3030
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 226 CGCG CG^CG
AcsI 382 AAATTC [GA]^AATT[CT]
AfaI 126 GTAC GT^AC
AflIII 320 ACGTGT A^C[GA][CT]GT
AlwI 282 GATCC GGATC(4/5)
AocI 234 CCTGAGG CC^T[ACGT]AGG
ApaI 308 GGGCCC GGGCC^C
ApoI 382 AAATTC [GA]^AATT[CT]
AvaI 311 CCCGGG C^[CT]CG[GA]G
AvaII 96 GGACC G^G[AT]CC
BbsI 136 GAAGAC GAAGAC(2/6)
BglI 167 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 136 GAAGAC GAAGAC(2/6)
BsiYI 171 CCTGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 171 CCTGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 377 GAGAC GTCTC(1/5)
BsmFI 314 GGGAC GGGAC(10/14)
BsoBI 311 CCCGGG C^[CT]CG[GA]G
BsrI 128 ACTGG ACTGG(1/-1)
BstF5I 213 CATCC GGATG(2/0)
BstNI 171 CCTGG CC^[AT]GG
BstUI 226 CGCG CG^CG
Bsu36I 234 CCTGAGG CC^T[ACGT]AGG
DdeI 235 CTGAG C^T[ACGT]AG
Eco81I 234 CCTGAGG CC^T[ACGT]AGG
EcoNI 171 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 171 CCTGG ^CC[AT]GG
EcoT14I 200 CCAAGG C^C[AT][AT]GG
FokI 213 CATCC GGATG(9/13)
HapII 312 CCGG C^CGG
HgaI 224 GACGC GACGC(5/10)
HinfI 454 GAATC G^A[ACGT]TC
HpaII 312 CCGG C^CGG
MaeII 320 ACGT A^CGT
MspI 312 CCGG C^CGG
MvaI 171 CCTGG CC^[AT]GG
MvnI 226 CGCG CG^CG
NlaIII 402 CATG CATG^
RsaI 126 GTAC GT^AC
ScaI 125 AGTACT AGT^ACT
SmaI 311 CCCGGG CCC^GGG
SmlI 145 CTCAAG C^T[CT][GA]AG
StyI 200 CCAAGG C^C[AT][AT]GG
TfiI 454 GAATC G^A[AT]TC
Tsp45I 161 GTGAC ^GT[GC]AC
Tsp509I 383 AATT ^AATT
XmaCI 311 CCCGGG C^CCGGG
XmaI 311 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BanI 247 GGCACC G^G[CT][GA]CC
445 GGCACC
BanII 183 GGGCTC G[GA]GC[CT]^C
308 GGGCCC
BcnI 311 CCCGG CC^[GC]GG
312 CCGGG
BmyI 183 GGGCTC G[AGT]GC[ACT]^C
308 GGGCCC
Bsp1286I 183 GGGCTC G[AGT]GC[ACT]^C
308 GGGCCC
FauI 73 GCGGG CCCGC(4/6)
306 GCGGG
HaeIII 204 GGCC GG^CC
309 GGCC
HphI 300 GGTGA GGTGA(8/7)
327 TCACC
MaeIII 80 GTAAC ^GT[ACGT]AC
161 GTGAC
NciI 311 CCCGG CC^[GC]GG
312 CCGGG
SfaNI 33 GATGC GCATC(5/9)
303 GATGC
TaqI 293 TCGA T^CGA
332 TCGA
TthHB8I 293 TCGA T^CGA
332 TCGA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
Cac8I Cfr13I DpnII Fnu4HI ItaI
MboI MboII MnlI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI