Restriction map for
pFN21AE1611
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI TfiI Tru9I
TseI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AluI 159 AGCT AG^CT
Alw44I 273 GTGCAC G^TGCAC
AlwI 292 GATCC GGATC(4/5)
AlwNI 97 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 273 GTGCAC G^TGCAC
AspHI 273 GTGCAC G[AT]GC[AT]^C
BbsI 15 GAAGAC GAAGAC(2/6)
BclI 110 TGATCA T^GATCA
BfaI 47 CTAG C^TAG
BpmI 31 CTGGAG CTGGAG(16/14)
BpuAI 15 GAAGAC GAAGAC(2/6)
BsaJI 84 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 273 GTGCAC G[AT]GC[AT]^C
BstAPI 245 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 18 GACGAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 5 TGGCCG [CT]^GGCC[GA]
FbaI 110 TGATCA T^GATCA
Fnu4HI 7 GCCGC GC^[ACGT]GC
HaeIII 6 GGCC GG^CC
ItaI 7 GCCGC GC^[ACGT]GC
MaeI 47 CTAG C^TAG
MaeII 80 ACGT A^CGT
MaeIII 299 GTGAC ^GT[ACGT]AC
MnlI 165 GAGG CCTC(7/6)
MslI 217 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 50 GGCTCC GG[ACGT]^[ACGT]CC
ScaI 182 AGTACT AGT^ACT
SspI 232 AATATT AAT^ATT
TaqI 37 TCGA T^CGA
Tsp45I 299 GTGAC ^GT[GC]AC
Tsp509I 131 AATT ^AATT
TthHB8I 37 TCGA T^CGA
XcmI 213 CCATCACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 8 CCGC CCGC(-3/-1)
254 GCGG
BmyI 243 GGGCAC G[AGT]GC[ACT]^C
273 GTGCAC
Bsp1286I 243 GGGCAC G[AGT]GC[ACT]^C
273 GTGCAC
BssKI 84 CCTGG ^CC[ACGT]GG
295 CCAGG
BstNI 84 CCTGG CC^[AT]GG
295 CCAGG
Cac8I 250 GCATGC GC[ACGT]^[ACGT]GC
320 GCATGC
EcoRII 84 CCTGG ^CC[AT]GG
295 CCAGG
HphI 298 GGTGA GGTGA(8/7)
337 GGTGA
MboII 15 GAAGA GAAGA(8/7)
105 GAAGA
MvaI 84 CCTGG CC^[AT]GG
295 CCAGG
MwoI 190 GCTATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
245 GCACAGCATGC
NlaIII 251 CATG CATG^
321 CATG
NspI 250 GCATGC [GA]CATG^[CT]
320 GCATGC
ScrFI 84 CCTGG CC^[ACGT]GG
295 CCAGG
SfcI 219 CTACAG C^T[GA][CT]AG
264 CTACAG
SphI 250 GCATGC GCATG^C
320 GCATGC
TspRI 222 CAGTG CA[GC]TG[ACGT][ACGT]^
278 CACTG
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AfaI BsrI DpnII HinfI MboI
NdeII PleI RsaI Sau3AI