##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AciI          312  GCGG                 CCGC(-3/-1)
AcsI          323  GAATTC               [GA]^AATT[CT]
ApoI          323  GAATTC               [GA]^AATT[CT]
AvaI          339  CTCGGG               C^[CT]CG[GA]G
BalI           35  TGGCCA               TGG^CCA
BanI          159  GGCACC               G^G[CT][GA]CC
BmyI          158  GGGCAC               G[AGT]GC[ACT]^C
BseRI          69  CTCCTC               GAGGAG(10/8)
BsgI          121  GTGCAG               GTGCAG(16/14)
BsoBI         339  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      158  GGGCAC               G[AGT]GC[ACT]^C
EaeI           35  TGGCCA               [CT]^GGCC[GA]
EarI          318  GAAGAG               CTCTTC(1/4)
EcoRI         323  GAATTC               G^AATTC
HphI          362  TCACC                GGTGA(8/7)
Ksp632I       318  GAAGAG               CTCTTC(1/4)
MluNI          35  TGGCCA               TGG^CCA
MscI           35  TGGCCA               TGG^CCA
MseI          251  TTAA                 T^TAA
MspA1I        133  CAGCTG               C[AC]G^C[GT]G
NlaIII         54  CATG                 CATG^
NlaIV         159  GGCACC               GG[ACGT]^[ACGT]CC
NspI           53  GCATGC               [GA]CATG^[CT]
PleI          150  GAGTC                GAGTC(4/5)
PvuII         133  CAGCTG               CAG^CTG
SfaNI         273  GCATC                GCATC(5/9)
SmlI          127  CTTGAG               C^T[CT][GA]AG
SphI           53  GCATGC               GCATG^C
Tru9I         251  TTAA                 T^TAA
Tsp509I       324  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI           97  GATCC                GGATC(4/5)
              372  GGATC               
AocI           71  CCTCAGG              CC^T[ACGT]AGG
              178  CCTAAGG             
BpmI          222  CTCCAG               CTGGAG(16/14)
              266  CTCCAG              
BsaJI         386  CCTGGG               C^C[ACGT][ACGT]GG
              415  CCCAGG              
Bsu36I         71  CCTCAGG              CC^T[ACGT]AGG
              178  CCTAAGG             
Cfr13I        413  GGCCC                G^G[ACGT]CC
              444  GGCCC               
DpnII          97  GATC                 ^GATC
              373  GATC                
Eco81I         71  CCTCAGG              CC^T[ACGT]AGG
              178  CCTAAGG             
MboI           97  GATC                 ^GATC
              373  GATC                
NdeII          97  GATC                 ^GATC
              373  GATC                
PstI          328  CTGCAG               CTGCA^G
              350  CTGCAG              
Sau3AI         97  GATC                 ^GATC
              373  GATC                
Sau96I        413  GGCCC                G^G[ACGT]CC
              444  GGCCC               
TfiI           63  GATTC                G^A[AT]TC
              450  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MboII       MnlI        MvaI        
MwoI        ScrFI       SfcI        TseI        TspRI