Restriction map for
pFN21AE5568
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI BalI BamHI BbsI
BbvCI BciVI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI HindIII HpaI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 103 GGTACC G^GTACC
AccII 584 CGCG CG^CG
AclI 383 AACGTT AA^CGTT
AflIII 511 ACGTGT A^C[GA][CT]GT
AocI 130 CCTGAGG CC^T[ACGT]AGG
ApaI 764 GGGCCC GGGCC^C
Asp718I 103 GGTACC G^GTACC
AviII 88 TGCGCA TGC^GCA
AvrII 63 CCTAGG C^CTAGG
BbeI 504 GGCGCC GGCGC^C
BcgI 1066 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 845 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 63 CCTAGG C^CTAGG
BseRI 696 CTCCTC GAGGAG(10/8)
BsgI 1064 GTGCAG GTGCAG(16/14)
BspHI 543 TCATGA T^CATGA
BsrBI 905 CCGCTC CCGCTC(-3/-3)
BsrFI 731 GCCGGC [GA]^CCGG[CT]
BssSI 574 CACGAG CACGAG(-5/-1)
BstUI 584 CGCG CG^CG
BstYI 626 AGATCC [GA]^GATC[CT]
Bsu36I 130 CCTGAGG CC^T[ACGT]AGG
Cfr10I 731 GCCGGC [GA]^CCGG[CT]
EagI 238 CGGCCG C^GGCCG
EclXI 238 CGGCCG C^GGCCG
Eco52I 238 CGGCCG C^GGCCG
Eco81I 130 CCTGAGG CC^T[ACGT]AGG
EcoT22I 553 ATGCAT ATGCA^T
FspI 88 TGCGCA TGC^GCA
HgaI 681 GACGC GACGC(5/10)
HincII 613 GTCAAC GT[CT]^[GA]AC
HindII 613 GTCAAC GT[CT]^[GA]AC
KasI 504 GGCGCC G^GCGCC
KpnI 103 GGTACC GGTAC^C
MaeIII 406 GTCAC ^GT[ACGT]AC
MflI 626 AGATCC [GA]^GATC[CT]
MseI 173 TTAA T^TAA
MvnI 584 CGCG CG^CG
NaeI 731 GCCGGC GCC^GGC
NarI 504 GGCGCC GG^CGCC
NcoI 284 CCATGG C^CATGG
NgoMIV 731 GCCGGC G^CCGGC
NsiI 553 ATGCAT ATGCA^T
PaeR7I 462 CTCGAG C^TCGAG
PshAI 254 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 383 AACGTT AA^CGTT
PvuII 84 CAGCTG CAG^CTG
RcaI 543 TCATGA T^CATGA
SacI 547 GAGCTC GAGCT^C
SbfI 1006 CCTGCAGG CCTGCA^GG
SfoI 504 GGCGCC GGC^GCC
SmaI 818 CCCGGG CCC^GGG
SmlI 462 CTCGAG C^T[CT][GA]AG
Sse8387I 1006 CCTGCAGG CCTGCA^GG
Tru9I 173 TTAA T^TAA
Tsp45I 406 GTCAC ^GT[GC]AC
Tsp509I 171 AATT ^AATT
XhoI 462 CTCGAG C^TCGAG
XhoII 626 AGATCC [GA]^GATC[CT]
XmaCI 818 CCCGGG C^CCGGG
XmaI 818 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 504 GGCGCC G[GA]^CG[CT]C
681 GACGCC
AlwI 60 GATCC GGATC(4/5)
627 GATCC
AspHI 547 GAGCTC G[AT]GC[AT]^C
577 GAGCAC
AvaI 462 CTCGAG C^[CT]CG[GA]G
818 CCCGGG
BbrPI 390 CACGTG CAC^GTG
510 CACGTG
BfaI 64 CTAG C^TAG
1262 CTAG
BsaAI 390 CACGTG [CT]AC^GT[GA]
510 CACGTG
BsaHI 504 GGCGCC G[GA]^CG[CT]C
681 GACGCC
BsaI 16 GGTCTC GGTCTC(1/5)
495 GGTCTC
BsiEI 238 CGGCCG CG[GA][CT]^CG
591 CGACCG
BsiHKAI 547 GAGCTC G[AT]GC[AT]^C
577 GAGCAC
BsoBI 462 CTCGAG C^[CT]CG[GA]G
818 CCCGGG
BspMI 489 GCAGGT ACCTGC(4/8)
1126 GCAGGT
BstXI 885 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1027 CCACATGGGTGG
DsaI 284 CCATGG C^C[GA][CT]GG
864 CCGTGG
HaeII 69 AGCGCC [GA]GCGC^[CT]
504 GGCGCC
Hin1I 504 GGCGCC G[GA]^CG[CT]C
681 GACGCC
MaeI 64 CTAG C^TAG
1262 CTAG
MspA1I 84 CAGCTG C[AC]G^C[GT]G
928 CAGCGG
NspI 555 GCATGT [GA]CATG^[CT]
674 ACATGC
PleI 243 GAGTC GAGTC(4/5)
1039 GAGTC
PmaCI 390 CACGTG CAC^GTG
510 CACGTG
PmlI 390 CACGTG CAC^GTG
510 CACGTG
TaqI 221 TCGA T^CGA
463 TCGA
TfiI 955 GAATC G^A[AT]TC
1208 GAATC
TthHB8I 221 TCGA T^CGA
463 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TseI TspRI