Restriction map for
pFN21AE1230
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AgeI 123 ACCGGT A^CCGGT
AlwNI 673 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 692 GAGCTC G[AT]GC[AT]^C
AspI 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 420 CCCGGG C^[CT]CG[GA]G
BamHI 362 GGATCC G^GATCC
BanI 577 GGCACC G^G[CT][GA]CC
BbrPI 517 CACGTG CAC^GTG
BglI 569 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 493 CTCCAG CTGGAG(16/14)
BsaAI 517 CACGTG [CT]AC^GT[GA]
BsaWI 123 ACCGGT [AT]^CCGG[AT]
BsiHKAI 692 GAGCTC G[AT]GC[AT]^C
BsmAI 902 GAGAC GTCTC(1/5)
BsoBI 420 CCCGGG C^[CT]CG[GA]G
BspHI 854 TCATGA T^CATGA
BstEII 720 GGTCACC G^GT[ACGT]ACC
BstPI 720 GGTCACC G^GT[ACGT]ACC
CfoI 347 GCGC GCG^C
DdeI 178 CTCAG C^T[ACGT]AG
DraIII 834 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 571 CGGCCG C^GGCCG
EclXI 571 CGGCCG C^GGCCG
Eco52I 571 CGGCCG C^GGCCG
EcoO65I 720 GGTCACC G^GT[ACGT]ACC
EcoT14I 32 CCATGG C^C[AT][AT]GG
HaeII 346 AGCGCC [GA]GCGC^[CT]
HhaI 347 GCGC GCG^C
HinP1I 347 GCGC G^CGC
MseI 959 TTAA T^TAA
MspA1I 88 CAGCGG C[AC]G^C[GT]G
NaeI 569 GCCGGC GCC^GGC
NcoI 32 CCATGG C^CATGG
NgoMIV 569 GCCGGC G^CCGGC
PflFI 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 123 ACCGGT A^CCGGT
PleI 937 GACTC GAGTC(4/5)
PmaCI 517 CACGTG CAC^GTG
PmlI 517 CACGTG CAC^GTG
PpuMI 165 GGGACCC [GA]G^G[AT]CC[CT]
PstI 553 CTGCAG CTGCA^G
RcaI 854 TCATGA T^CATGA
SacI 692 GAGCTC GAGCT^C
SbfI 552 CCTGCAGG CCTGCA^GG
SmaI 420 CCCGGG CCC^GGG
SmlI 1021 CTCAAG C^T[CT][GA]AG
Sse8387I 552 CCTGCAGG CCTGCA^GG
StyI 32 CCATGG C^C[AT][AT]GG
Tru9I 959 TTAA T^TAA
Tsp45I 721 GTCAC ^GT[GC]AC
Tsp509I 922 AATT ^AATT
Tth111I 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 725 CCAGTAATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 420 CCCGGG C^CCGGG
XmaI 420 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BalI 254 TGGCCA TGG^CCA
917 TGGCCA
BanII 692 GAGCTC G[GA]GC[CT]^C
804 GGGCTC
BfaI 94 CTAG C^TAG
142 CTAG
BseRI 400 GAGGAG GAGGAG(10/8)
526 GAGGAG
BsiEI 571 CGGCCG CG[GA][CT]^CG
654 CGACCG
BspMI 810 ACCTGC ACCTGC(4/8)
555 GCAGGT
BstXI 628 CCAAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
908 CCATCTACCTGG
EcoNI 477 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
666 CCTACAGCAGG
MaeI 94 CTAG C^TAG
142 CTAG
MluNI 254 TGGCCA TGG^CCA
917 TGGCCA
MscI 254 TGGCCA TGG^CCA
917 TGGCCA
NheI 93 GCTAGC G^CTAGC
141 GCTAGC
SfaNI 15 GATGC GCATC(5/9)
294 GATGC
TfiI 102 GATTC G^A[AT]TC
323 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI TseI TspRI XhoII