Restriction map for
pFN21AE3481
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AvrII BfrI
BlnI BsiEI BsiWI BsmBI BspDI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI HindIII
HpaI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RsrII SacII
SapI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1660 GACGTC GACGT^C
AclI 2320 AACGTT AA^CGTT
AflIII 974 ACGTGT A^C[GA][CT]GT
ApaI 1769 GGGCCC GGGCC^C
BbeI 4948 GGCGCC GGCGC^C
BbrPI 2400 CACGTG CAC^GTG
BcgI 305 GCAACAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3929 GTATCC GTATCC(6/5)
BclI 1478 TGATCA T^GATCA
BlpI 903 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 903 GCTGAGC GC^T[ACGT]AGC
BsaBI 1035 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4631 TGTACA T^GTACA
BsrBI 378 GAGCGG CCGCTC(-3/-3)
BsrGI 4631 TGTACA T^GTACA
BstBI 2519 TTCGAA TT^CGAA
CelII 903 GCTGAGC GC^T[ACGT]AGC
DraIII 2397 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2936 GAATTC G^AATTC
FbaI 1478 TGATCA T^GATCA
KasI 4948 GGCGCC G^GCGCC
MamI 1035 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4948 GGCGCC GG^CGCC
NcoI 122 CCATGG C^CATGG
NdeI 2690 CATATG CA^TATG
NspV 2519 TTCGAA TT^CGAA
PmaCI 2400 CACGTG CAC^GTG
PmlI 2400 CACGTG CAC^GTG
Psp1406I 2320 AACGTT AA^CGTT
SalI 2983 GTCGAC G^TCGAC
SbfI 2718 CCTGCAGG CCTGCA^GG
ScaI 1085 AGTACT AGT^ACT
SfiI 3423 GGCCCTCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4948 GGCGCC GGC^GCC
SfuI 2519 TTCGAA TT^CGAA
SgrAI 1905 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 1382 TACGTA TAC^GTA
SpeI 198 ACTAGT A^CTAGT
SphI 2090 GCATGC GCATG^C
Sse8387I 2718 CCTGCAGG CCTGCA^GG
SspBI 4631 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 383 GGTACC G^GTACC
4172 GGTACC
AccIII 585 TCCGGA T^CCGGA
4482 TCCGGA
AhdI 532 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACACCGAGTC
AocI 11 CCTCAGG CC^T[ACGT]AGG
4129 CCTCAGG
Asp700I 2163 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4548 GAATTGCTTC
Asp718I 383 GGTACC G^GTACC
4172 GGTACC
AspEI 532 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACACCGAGTC
AviII 1271 TGCGCA TGC^GCA
2069 TGCGCA
BbvCI 4851 CCTCAGC CCTCAGC(-5/-2)
4540 GCTGAGG
BglII 1844 AGATCT A^GATCT
4160 AGATCT
BseAI 585 TCCGGA T^CCGGA
4482 TCCGGA
BspEI 585 TCCGGA T^CCGGA
4482 TCCGGA
BspHI 1481 TCATGA T^CATGA
2369 TCATGA
BsrDI 2265 CATTGC GCAATG(2/0)
3570 CATTGC
BstAPI 1323 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2072 GCAAGTGCTGC
Bsu36I 11 CCTCAGG CC^T[ACGT]AGG
4129 CCTCAGG
Eam1105I 532 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACACCGAGTC
Eco81I 11 CCTCAGG CC^T[ACGT]AGG
4129 CCTCAGG
FspI 1271 TGCGCA TGC^GCA
2069 TGCGCA
HaeII 2123 AGCGCC [GA]GCGC^[CT]
4948 GGCGCC
KpnI 383 GGTACC GGTAC^C
4172 GGTACC
MroI 585 TCCGGA T^CCGGA
4482 TCCGGA
NspI 2090 GCATGC [GA]CATG^[CT]
2423 GCATGT
RcaI 1481 TCATGA T^CATGA
2369 TCATGA
StuI 4126 AGGCCT AGG^CCT
4604 AGGCCT
XmnI 2163 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4548 GAATTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI