Restriction map for
pFN21AE6165
##################################
# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 103 GTCTAC GT^[AC][GT]AC
AciI 348 CCGC CCGC(-3/-1)
AcsI 364 GAATTC [GA]^AATT[CT]
AfaI 435 GTAC GT^AC
ApoI 364 GAATTC [GA]^AATT[CT]
AvaII 113 GGACC G^G[AT]CC
BamHI 297 GGATCC G^GATCC
BanI 208 GGTGCC G^G[CT][GA]CC
BbrPI 292 CACGTG CAC^GTG
BbsI 24 GAAGAC GAAGAC(2/6)
BfaI 205 CTAG C^TAG
BmyI 249 GGGCAC G[AGT]GC[ACT]^C
BpuAI 24 GAAGAC GAAGAC(2/6)
BsaAI 292 CACGTG [CT]AC^GT[GA]
BseRI 19 GAGGAG GAGGAG(10/8)
Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C
BsrDI 323 GCAATG GCAATG(2/0)
BsrI 96 ACTGG ACTGG(1/-1)
BstF5I 63 GGATG GGATG(2/0)
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 364 GAATTC G^AATTC
EcoT14I 109 CCTTGG C^C[AT][AT]GG
FauI 347 CCCGC CCCGC(4/6)
FokI 63 GGATG GGATG(9/13)
MaeI 205 CTAG C^TAG
MaeIII 281 GTGAC ^GT[ACGT]AC
MboII 24 GAAGA GAAGA(8/7)
MspA1I 262 CAGCTG C[AC]G^C[GT]G
NlaIII 190 CATG CATG^
PmaCI 292 CACGTG CAC^GTG
PmlI 292 CACGTG CAC^GTG
PshAI 27 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 262 CAGCTG CAG^CTG
RsaI 435 GTAC GT^AC
SmlI 244 CTCAAG C^T[CT][GA]AG
StyI 109 CCTTGG C^C[AT][AT]GG
Tsp45I 281 GTGAC ^GT[GC]AC
Tsp509I 365 AATT ^AATT
TspRI 277 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AocI 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
BsgI 274 CTGCAC GTGCAG(16/14)
289 CTGCAC
BstYI 141 GGATCT [GA]^GATC[CT]
297 GGATCC
Bsu36I 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
DpnII 142 GATC ^GATC
298 GATC
Eco81I 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
HinfI 12 GACTC G^A[ACGT]TC
130 GAGTC
MaeII 59 ACGT A^CGT
293 ACGT
MboI 142 GATC ^GATC
298 GATC
MflI 141 GGATCT [GA]^GATC[CT]
297 GGATCC
NdeII 142 GATC ^GATC
298 GATC
PleI 12 GACTC GAGTC(4/5)
130 GAGTC
Sau3AI 142 GATC ^GATC
298 GATC
XhoII 141 GGATCT [GA]^GATC[CT]
297 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII HphI ItaI MnlI
MvaI MwoI NlaIV Sau96I ScrFI
SfaNI TseI