Restriction map for
pFN21AE2013
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SmaI SmlI SnaBI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1174 GTCTAC GT^[AC][GT]AC
AcyI 30 GGCGCC G[GA]^CG[CT]C
AflIII 223 ACATGT A^C[GA][CT]GT
Alw44I 380 GTGCAC G^TGCAC
ApaI 561 GGGCCC GGGCC^C
ApaLI 380 GTGCAC G^TGCAC
BalI 524 TGGCCA TGG^CCA
BamHI 1582 GGATCC G^GATCC
BanII 561 GGGCCC G[GA]GC[CT]^C
BbeI 30 GGCGCC GGCGC^C
BclI 573 TGATCA T^GATCA
BglII 1002 AGATCT A^GATCT
BsaHI 30 GGCGCC G[GA]^CG[CT]C
BsgI 1366 GTGCAG GTGCAG(16/14)
BsmBI 1358 GAGACG CGTCTC(1/5)
BspLU11I 223 ACATGT A^CATGT
BspMI 589 GCAGGT ACCTGC(4/8)
BsrFI 33 GCCGGT [GA]^CCGG[CT]
BssSI 621 CACGAG CACGAG(-5/-1)
BstXI 1524 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 31 GCGC GCG^C
Cfr10I 33 GCCGGT [GA]^CCGG[CT]
DraIII 1556 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 524 TGGCCA [CT]^GGCC[GA]
EcoNI 1341 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 603 CCCGC CCCGC(4/6)
FbaI 573 TGATCA T^GATCA
HaeII 30 GGCGCC [GA]GCGC^[CT]
HapII 34 CCGG C^CGG
HgaI 1360 GACGC GACGC(5/10)
HhaI 31 GCGC GCG^C
Hin1I 30 GGCGCC G[GA]^CG[CT]C
HinP1I 31 GCGC G^CGC
HincII 127 GTCAAC GT[CT]^[GA]AC
HindII 127 GTCAAC GT[CT]^[GA]AC
HpaII 34 CCGG C^CGG
KasI 30 GGCGCC G^GCGCC
MluNI 524 TGGCCA TGG^CCA
MscI 524 TGGCCA TGG^CCA
MspI 34 CCGG C^CGG
NarI 30 GGCGCC GG^CGCC
PflMI 11 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1535 GAGTC GAGTC(4/5)
PpuMI 1021 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 229 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 30 GGCGCC GGC^GCC
SgrAI 32 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 1416 ACTAGT A^CTAGT
SphI 879 GCATGC GCATG^C
SspI 786 AATATT AAT^ATT
StuI 1590 AGGCCT AGG^CCT
Van91I 11 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 30 GGCGCC G^G[CT][GA]CC
1159 GGTGCC
BsaI 440 GAGACC GGTCTC(1/5)
1605 GGTCTC
BsrDI 419 GCAATG GCAATG(2/0)
725 GCAATG
BstYI 1002 AGATCT [GA]^GATC[CT]
1582 GGATCC
DraI 772 TTTAAA TTT^AAA
1147 TTTAAA
DrdI 310 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACTTGGTGGTC
EarI 1006 CTCTTC CTCTTC(1/4)
1168 GAAGAG
EcoRI 6 GAATTC G^AATTC
682 GAATTC
HphI 94 GGTGA GGTGA(8/7)
1304 TCACC
Ksp632I 1006 CTCTTC CTCTTC(1/4)
1168 GAAGAG
MflI 1002 AGATCT [GA]^GATC[CT]
1582 GGATCC
MslI 1126 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1251 CACCATGGTG
NspI 223 ACATGT [GA]CATG^[CT]
879 GCATGC
SfcI 462 CTGTAG C^T[GA][CT]AG
1196 CTGTAG
TaqI 320 TCGA T^CGA
819 TCGA
TfiI 703 GAATC G^A[AT]TC
851 GATTC
TthHB8I 320 TCGA T^CGA
819 TCGA
XhoII 1002 AGATCT [GA]^GATC[CT]
1582 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI