Restriction map for
pFN21AE3322
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI BstEII BstPI BstUI
Bsu36I CelII CfoI ClaI CpoI
DraII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Alw44I 1519 GTGCAC G^TGCAC
ApaLI 1519 GTGCAC G^TGCAC
BbvCI 2254 CCTCAGC CCTCAGC(-5/-2)
BcgI 622 GCAGATTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2591 GTATCC GTATCC(6/5)
BpmI 2015 CTGGAG CTGGAG(16/14)
BsaAI 17 TACGTG [CT]AC^GT[GA]
BseRI 2719 GAGGAG GAGGAG(10/8)
BsmFI 451 GGGAC GGGAC(10/14)
BsrFI 2471 ACCGGC [GA]^CCGG[CT]
Bst1107I 1921 GTATAC GTA^TAC
BstAPI 2259 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1727 TTCGAA TT^CGAA
BstZ17I 1921 GTATAC GTA^TAC
Cfr10I 2471 ACCGGC [GA]^CCGG[CT]
Cfr13I 2601 GGCCC G^G[ACGT]CC
DraIII 828 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2727 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 2580 GCGGG CCCGC(4/6)
HapII 2472 CCGG C^CGG
HincII 2059 GTTGAC GT[CT]^[GA]AC
HindII 2059 GTTGAC GT[CT]^[GA]AC
HpaII 2472 CCGG C^CGG
MspA1I 1109 CAGCTG C[AC]G^C[GT]G
MspI 2472 CCGG C^CGG
NspI 2648 ACATGC [GA]CATG^[CT]
NspV 1727 TTCGAA TT^CGAA
PflMI 1798 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1112 CTGCAG CTGCA^G
PvuII 1109 CAGCTG CAG^CTG
SapI 1468 GAAGAGC GCTCTTC(1/4)
Sau96I 2601 GGCCC G^G[ACGT]CC
SexAI 333 ACCTGGT A^CC[AT]GGT
SfuI 1727 TTCGAA TT^CGAA
Van91I 1798 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1781 CCAAAGATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1440 GTCTAC GT^[AC][GT]AC
1921 GTATAC
AciI 506 GCGG CCGC(-3/-1)
2580 GCGG
AfaI 1881 GTAC GT^AC
2634 GTAC
AlwI 1270 GATCC GGATC(4/5)
1807 GGATC
AspHI 1498 GAGCAC G[AT]GC[AT]^C
1519 GTGCAC
BanI 1660 GGCACC G^G[CT][GA]CC
2290 GGCACC
BglII 2555 AGATCT A^GATCT
2660 AGATCT
BsaI 330 GAGACC GGTCTC(1/5)
2722 GAGACC
BsiHKAI 1498 GAGCAC G[AT]GC[AT]^C
1519 GTGCAC
BspHI 719 TCATGA T^CATGA
1149 TCATGA
BspMI 209 ACCTGC ACCTGC(4/8)
2025 ACCTGC
BstXI 1016 CCATGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2007 CCACAAAGCTGG
BstYI 2555 AGATCT [GA]^GATC[CT]
2660 AGATCT
DrdI 1606 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACTCTAAAGTC
EcoRV 54 GATATC GAT^ATC
1786 GATATC
EcoT22I 714 ATGCAT ATGCA^T
2650 ATGCAT
HindIII 1346 AAGCTT A^AGCTT
2274 AAGCTT
HphI 207 TCACC GGTGA(8/7)
1557 TCACC
MaeII 18 ACGT A^CGT
1975 ACGT
MfeI 277 CAATTG C^AATTG
661 CAATTG
MflI 2555 AGATCT [GA]^GATC[CT]
2660 AGATCT
MslI 1126 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1648 CATTGCAATG
MunI 277 CAATTG C^AATTG
661 CAATTG
NcoI 985 CCATGG C^CATGG
1125 CCATGG
NsiI 714 ATGCAT ATGCA^T
2650 ATGCAT
PshAI 453 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
751 GACATTGGTC
RcaI 719 TCATGA T^CATGA
1149 TCATGA
RsaI 1881 GTAC GT^AC
2634 GTAC
SspI 163 AATATT AAT^ATT
1628 AATATT
Tsp45I 818 GTGAC ^GT[GC]AC
2225 GTGAC
XhoII 2555 AGATCT [GA]^GATC[CT]
2660 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AluI AlwNI ApoI Asp700I
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII DraI
DsaI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI