Restriction map for
pFN21AE1639
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FokI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 196 GTCTAC GT^[AC][GT]AC
AccII 59 CGCG CG^CG
AfaI 346 GTAC GT^AC
AflIII 200 ACATGT A^C[GA][CT]GT
BclI 287 TGATCA T^GATCA
BcnI 450 CCCGG CC^[GC]GG
BglI 368 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 243 CTGGAG CTGGAG(16/14)
BsaAI 363 TACGTG [CT]AC^GT[GA]
BsaBI 111 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 241 CCCTGG C^C[ACGT][ACGT]GG
BseRI 147 CTCCTC GAGGAG(10/8)
BspLU11I 200 ACATGT A^CATGT
BspMI 209 ACCTGC ACCTGC(4/8)
BstUI 59 CGCG CG^CG
Cac8I 43 GCCTGC GC[ACGT]^[ACGT]GC
FauI 439 CCCGC CCCGC(4/6)
FbaI 287 TGATCA T^GATCA
HaeII 14 AGCGCC [GA]GCGC^[CT]
HapII 451 CCGG C^CGG
HpaII 451 CCGG C^CGG
MaeII 364 ACGT A^CGT
MamI 111 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 254 TCTTC GAAGA(8/7)
MspI 451 CCGG C^CGG
MvnI 59 CGCG CG^CG
MwoI 368 GCCTCTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 450 CCCGG CC^[GC]GG
NlaIII 201 CATG CATG^
NlaIV 24 GGCTCC GG[ACGT]^[ACGT]CC
NspI 200 ACATGT [GA]CATG^[CT]
RsaI 346 GTAC GT^AC
SfaNI 382 GATGC GCATC(5/9)
TaqI 66 TCGA T^CGA
TthHB8I 66 TCGA T^CGA
XcmI 226 CCATTCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 111 GATCC GGATC(4/5)
171 GATCC
BsgI 45 CTGCAC GTGCAG(16/14)
211 CTGCAC
BsiYI 264 CCTCATAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
439 CCCGCAGATGG
BslI 264 CCTCATAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
439 CCCGCAGATGG
BsrI 236 ACTGG ACTGG(1/-1)
348 ACTGG
CfoI 15 GCGC GCG^C
60 GCGC
DdeI 11 CTGAG C^T[ACGT]AG
89 CTGAG
HhaI 15 GCGC GCG^C
60 GCGC
HinP1I 15 GCGC G^CGC
60 GCGC
HinfI 7 GATTC G^A[ACGT]TC
74 GAATC
MaeIII 206 GTGAC ^GT[ACGT]AC
391 GTCAC
TfiI 7 GATTC G^A[AT]TC
74 GAATC
Tsp45I 206 GTGAC ^GT[GC]AC
391 GTCAC
TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^
432 CACTG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AciI BbvI BssKI BstNI Cfr13I
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MboI MnlI MvaI NdeII
Sau3AI Sau96I ScrFI TseI