Restriction map for
pFN21AE6567
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# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoT22I FbaI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AluI 7 AGCT AG^CT
BciVI 445 GTATCC GTATCC(6/5)
BpmI 345 CTCCAG CTGGAG(16/14)
BsiEI 313 CGGCCG CG[GA][CT]^CG
BsmFI 15 GGGAC GGGAC(10/14)
DdeI 123 CTCAG C^T[ACGT]AG
DpnII 382 GATC ^GATC
DraII 520 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 17 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 313 CGGCCG [CT]^GGCC[GA]
EagI 313 CGGCCG C^GGCCG
EclXI 313 CGGCCG C^GGCCG
Eco52I 313 CGGCCG C^GGCCG
EcoNI 319 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 520 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 127 GATATC GAT^ATC
EcoT14I 199 CCAAGG C^C[AT][AT]GG
FauI 294 CCCGC CCCGC(4/6)
HapII 239 CCGG C^CGG
HgaI 287 GACGC GACGC(5/10)
HinfI 171 GAATC G^A[ACGT]TC
HpaII 239 CCGG C^CGG
MaeII 394 ACGT A^CGT
MboI 382 GATC ^GATC
MspA1I 180 CAGCGG C[AC]G^C[GT]G
MspI 239 CCGG C^CGG
NdeII 382 GATC ^GATC
NlaIII 526 CATG CATG^
NlaIV 521 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 520 AGGACCC [GA]G^G[AT]CC[CT]
PstI 320 CTGCAG CTGCA^G
Sau3AI 382 GATC ^GATC
SbfI 319 CCTGCAGG CCTGCA^GG
SfaNI 85 GATGC GCATC(5/9)
SfcI 320 CTGCAG C^T[GA][CT]AG
Sse8387I 319 CCTGCAGG CCTGCA^GG
StyI 199 CCAAGG C^C[AT][AT]GG
TfiI 171 GAATC G^A[AT]TC
Tsp45I 460 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaI 56 CCCGAG C^[CT]CG[GA]G
166 CCCGAG
AvaII 273 GGACC G^G[AT]CC
521 GGACC
BbvI 475 GCAGC GCAGC(8/12)
478 GCAGC
BsmAI 68 GAGAC GTCTC(1/5)
435 GTCTC
BsoBI 56 CCCGAG C^[CT]CG[GA]G
166 CCCGAG
BsrI 347 CCAGT ACTGG(1/-1)
482 CCAGT
Cac8I 51 GCACGC GC[ACGT]^[ACGT]GC
318 GCCTGC
DsaI 310 CCACGG C^C[GA][CT]GG
449 CCGTGG
HphI 459 GGTGA GGTGA(8/7)
384 TCACC
MaeIII 257 GTTAC ^GT[ACGT]AC
460 GTGAC
TseI 475 GCAGC G^C[AT]GC
478 GCAGC
Tsp509I 235 AATT ^AATT
511 AATT
TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^
464 CACTG
XcmI 387 CCATTCAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
440 CCATCGTATCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
BstNI Cfr13I EcoRII Fnu4HI HaeIII
ItaI MnlI MvaI MwoI Sau96I
ScrFI