Restriction map for
pFN21AE9561
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApoI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NcoI NdeI NdeII NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 359 GGTACC G^GTACC
AccI 868 GTCTAC GT^[AC][GT]AC
Alw44I 292 GTGCAC G^TGCAC
AlwNI 56 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 292 GTGCAC G^TGCAC
AscI 701 GGCGCGCC GG^CGCGCC
Asp718I 359 GGTACC G^GTACC
AviII 398 TGCGCA TGC^GCA
BalI 557 TGGCCA TGG^CCA
BbeI 462 GGCGCC GGCGC^C
BbvCI 471 CCTCAGC CCTCAGC(-5/-2)
BfaI 1016 CTAG C^TAG
BpmI 505 CTGGAG CTGGAG(16/14)
BsaWI 1098 ACCGGA [AT]^CCGG[AT]
BsmBI 1004 GAGACG CGTCTC(1/5)
BsrBI 862 CCGCTC CCGCTC(-3/-3)
BsrDI 101 GCAATG GCAATG(2/0)
BsrFI 571 GCCGGC [GA]^CCGG[CT]
BsrI 24 ACTGG ACTGG(1/-1)
BstAPI 375 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 571 GCCGGC [GA]^CCGG[CT]
DdeI 472 CTCAG C^T[ACGT]AG
EarI 454 CTCTTC CTCTTC(1/4)
FspI 398 TGCGCA TGC^GCA
KasI 462 GGCGCC G^GCGCC
KpnI 359 GGTACC GGTAC^C
Ksp632I 454 CTCTTC CTCTTC(1/4)
KspI 650 CCGCGG CCGC^GG
MaeI 1016 CTAG C^TAG
MaeII 554 ACGT A^CGT
MaeIII 410 GTAAC ^GT[ACGT]AC
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
NaeI 571 GCCGGC GCC^GGC
NarI 462 GGCGCC GG^CGCC
NgoMIV 571 GCCGGC G^CCGGC
NotI 923 GCGGCCGC GC^GGCCGC
PstI 127 CTGCAG CTGCA^G
SacI 508 GAGCTC GAGCT^C
SacII 650 CCGCGG CCGC^GG
SapI 453 GCTCTTC GCTCTTC(1/4)
SfcI 127 CTGCAG C^T[GA][CT]AG
SfoI 462 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BglI 706 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
986 GCCCACCCGGC
BsmAI 1004 GAGAC GTCTC(1/5)
346 GTCTC
BsmFI 1065 GGGAC GGGAC(10/14)
647 GTCCC
BspMI 296 ACCTGC ACCTGC(4/8)
578 ACCTGC
HaeII 450 GGCGCT [GA]GCGC^[CT]
462 GGCGCC
HphI 597 GGTGA GGTGA(8/7)
311 TCACC
MboII 80 TCTTC GAAGA(8/7)
455 TCTTC
MspA1I 379 CCGCTG C[AC]G^C[GT]G
650 CCGCGG
NlaIII 315 CATG CATG^
708 CATG
PpuMI 645 GGGTCCC [GA]G^G[AT]CC[CT]
1065 GGGACCC
SfaNI 7 GATGC GCATC(5/9)
256 GATGC
XcmI 242 CCATCTACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
707 CCATGCTGGCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV RsaI Sau96I ScrFI TseI
TspRI