Restriction map for
pFN21AE4118
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI ApoI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI ScaI SfaNI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 7 GACGTC GACGT^C
Alw44I 1303 GTGCAC G^TGCAC
Aor51HI 138 AGCGCT AGC^GCT
ApaLI 1303 GTGCAC G^TGCAC
AscI 766 GGCGCGCC GG^CGCGCC
BalI 1034 TGGCCA TGG^CCA
BbrPI 1294 CACGTG CAC^GTG
BbsI 979 GAAGAC GAAGAC(2/6)
BfaI 196 CTAG C^TAG
BglI 649 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1235 AGATCT A^GATCT
BlpI 1443 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1443 GCTCAGC GC^T[ACGT]AGC
BpuAI 979 GAAGAC GAAGAC(2/6)
BsaAI 1294 CACGTG [CT]AC^GT[GA]
BsaI 1280 GAGACC GGTCTC(1/5)
BsmBI 814 GAGACG CGTCTC(1/5)
BsrDI 464 GCAATG GCAATG(2/0)
BssSI 1357 CACGAG CACGAG(-5/-1)
BstF5I 3 GGATG GGATG(2/0)
BstYI 1235 AGATCT [GA]^GATC[CT]
CelII 1443 GCTCAGC GC^T[ACGT]AGC
CpoI 866 CGGACCG CG^G[AT]CCG
DpnII 1236 GATC ^GATC
Eco47III 138 AGCGCT AGC^GCT
FokI 3 GGATG GGATG(9/13)
HinfI 268 GACTC G^A[ACGT]TC
MaeI 196 CTAG C^TAG
MaeIII 1040 GTCAC ^GT[ACGT]AC
MboI 1236 GATC ^GATC
MflI 1235 AGATCT [GA]^GATC[CT]
MluNI 1034 TGGCCA TGG^CCA
MscI 1034 TGGCCA TGG^CCA
NaeI 502 GCCGGC GCC^GGC
NdeII 1236 GATC ^GATC
NgoMIV 502 GCCGGC G^CCGGC
NspI 154 GCATGT [GA]CATG^[CT]
PleI 268 GACTC GAGTC(4/5)
PmaCI 1294 CACGTG CAC^GTG
PmlI 1294 CACGTG CAC^GTG
RsrII 866 CGGACCG CG^G[AT]CCG
SacI 337 GAGCTC GAGCT^C
Sau3AI 1236 GATC ^GATC
SbfI 753 CCTGCAGG CCTGCA^GG
SexAI 290 ACCAGGT A^CC[AT]GGT
SgrAI 1126 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 753 CCTGCAGG CCTGCA^GG
TaqI 626 TCGA T^CGA
Tsp45I 1040 GTCAC ^GT[GC]AC
TthHB8I 626 TCGA T^CGA
XcmI 775 CCAGCGCAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1235 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 990 GTAC GT^AC
1098 GTAC
BsmAI 814 GAGAC GTCTC(1/5)
1280 GAGAC
BssHII 614 GCGCGC G^CGCGC
767 GCGCGC
BstXI 384 CCAGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1037 CCAGTCACCTGG
EcoT14I 254 CCAAGG C^C[AT][AT]GG
1115 CCTTGG
NlaIII 155 CATG CATG^
1482 CATG
PpuMI 59 GGGTCCC [GA]G^G[AT]CC[CT]
1006 GGGACCT
PvuII 546 CAGCTG CAG^CTG
569 CAGCTG
RsaI 990 GTAC GT^AC
1098 GTAC
SmaI 762 CCCGGG CCC^GGG
1178 CCCGGG
StyI 254 CCAAGG C^C[AT][AT]GG
1115 CCTTGG
XmaCI 762 CCCGGG C^CCGGG
1178 CCCGGG
XmaI 762 CCCGGG C^CCGGG
1178 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
Asp700I AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
KspI MaeII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIV NotI PstI
SacII Sau96I ScrFI SfcI SfoI
SmlI TseI TspRI XmnI