Restriction map for
pFN21AE6893
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI Asp700I Asp718I AspHI
AviII BamHI BbeI BbsI BciVI
BclI BfrI BpmI BpuAI BsaBI
BsaI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco81I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccIII 8 TCCGGA T^CCGGA
AcyI 646 GGCGTC G[GA]^CG[CT]C
AhdI 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 992 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 689 AGCGCT AGC^GCT
ApaI 186 GGGCCC GGGCC^C
AseI 1054 ATTAAT AT^TAAT
AsnI 1054 ATTAAT AT^TAAT
AspEI 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1039 CCCGAG C^[CT]CG[GA]G
AvaII 624 GGACC G^G[AT]CC
AvrII 105 CCTAGG C^CTAGG
BanII 186 GGGCCC G[GA]GC[CT]^C
BbrPI 652 CACGTG CAC^GTG
BbvCI 685 CCTCAGC CCTCAGC(-5/-2)
BcgI 635 GCACCTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1010 AGATCT A^GATCT
BlnI 105 CCTAGG C^CTAGG
BlpI 1087 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1087 GCTAAGC GC^T[ACGT]AGC
BsaAI 652 CACGTG [CT]AC^GT[GA]
BsaHI 646 GGCGTC G[GA]^CG[CT]C
BsaWI 8 TCCGGA [AT]^CCGG[AT]
BseAI 8 TCCGGA T^CCGGA
BsgI 559 CTGCAC GTGCAG(16/14)
BsiEI 318 CGGCCG CG[GA][CT]^CG
BsoBI 1039 CCCGAG C^[CT]CG[GA]G
BspEI 8 TCCGGA T^CCGGA
BspMI 675 GCAGGT ACCTGC(4/8)
BsrFI 260 GCCGGT [GA]^CCGG[CT]
BstEII 777 GGTCACC G^GT[ACGT]ACC
BstF5I 446 GGATG GGATG(2/0)
BstPI 777 GGTCACC G^GT[ACGT]ACC
BstXI 290 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1087 GCTAAGC GC^T[ACGT]AGC
Cfr10I 260 GCCGGT [GA]^CCGG[CT]
DraII 186 GGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 318 CGGCCG C^GGCCG
Eam1105I 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 318 CGGCCG C^GGCCG
Eco47III 689 AGCGCT AGC^GCT
Eco52I 318 CGGCCG C^GGCCG
EcoNI 723 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 186 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 777 GGTCACC G^GT[ACGT]ACC
EcoT22I 346 ATGCAT ATGCA^T
FokI 446 GGATG GGATG(9/13)
HgaI 647 GCGTC GACGC(5/10)
Hin1I 646 GGCGTC G[GA]^CG[CT]C
HinfI 994 GACTC G^A[ACGT]TC
MroI 8 TCCGGA T^CCGGA
MslI 906 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 734 CCATGG C^CATGG
NsiI 346 ATGCAT ATGCA^T
NspI 348 GCATGC [GA]CATG^[CT]
PflFI 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 734 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 994 GACTC GAGTC(4/5)
PmaCI 652 CACGTG CAC^GTG
PmlI 652 CACGTG CAC^GTG
PshBI 1054 ATTAAT AT^TAAT
SfaNI 374 GCATC GCATC(5/9)
SmlI 468 CTTGAG C^T[CT][GA]AG
SphI 348 GCATGC GCATG^C
StuI 108 AGGCCT AGG^CCT
Tth111I 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 734 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 247 GTCTAC GT^[AC][GT]AC
967 GTCTAC
AccII 462 CGCG CG^CG
632 CGCG
AfaI 176 GTAC GT^AC
459 GTAC
BalI 155 TGGCCA TGG^CCA
974 TGGCCA
BanI 235 GGCACC G^G[CT][GA]CC
922 GGCACC
BfaI 43 CTAG C^TAG
106 CTAG
BglI 352 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
943 GCCTACCTGGC
BmyI 186 GGGCCC G[AGT]GC[ACT]^C
749 GGGCAC
BseRI 198 CTCCTC GAGGAG(10/8)
454 GAGGAG
BsmAI 959 GAGAC GTCTC(1/5)
1068 GTCTC
Bsp1286I 186 GGGCCC G[AGT]GC[ACT]^C
749 GGGCAC
BstAPI 585 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
675 GCAGGTTATGC
BstUI 462 CGCG CG^CG
632 CGCG
DraIII 414 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
891 CACACAGTG
EarI 201 CTCTTC CTCTTC(1/4)
577 GAAGAG
EcoT14I 105 CCTAGG C^C[AT][AT]GG
734 CCATGG
FauI 630 CCCGC CCCGC(4/6)
423 GCGGG
HaeII 689 AGCGCT [GA]GCGC^[CT]
715 AGCGCC
Ksp632I 201 CTCTTC CTCTTC(1/4)
577 GAAGAG
MaeI 43 CTAG C^TAG
106 CTAG
MaeII 653 ACGT A^CGT
838 ACGT
MluNI 155 TGGCCA TGG^CCA
974 TGGCCA
MscI 155 TGGCCA TGG^CCA
974 TGGCCA
MseI 1016 TTAA T^TAA
1055 TTAA
MvnI 462 CGCG CG^CG
632 CGCG
PstI 430 CTGCAG CTGCA^G
673 CTGCAG
RsaI 176 GTAC GT^AC
459 GTAC
SfcI 430 CTGCAG C^T[GA][CT]AG
673 CTGCAG
StyI 105 CCTAGG C^C[AT][AT]GG
734 CCATGG
TaqI 115 TCGA T^CGA
815 TCGA
Tru9I 1016 TTAA T^TAA
1055 TTAA
TthHB8I 115 TCGA T^CGA
815 TCGA
XcmI 651 CCACGTGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
782 CCAGCAACGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI XhoII