Restriction map for
pFN21AE4301
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HgaI HindIII HpaI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 799 GTCTAC GT^[AC][GT]AC
AhdI 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 110 GGATC GGATC(4/5)
AspEI 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 706 GAGCAC G[AT]GC[AT]^C
AvaI 826 CCCGAG C^[CT]CG[GA]G
BalI 380 TGGCCA TGG^CCA
BanII 847 GAGCCC G[GA]GC[CT]^C
BbsI 393 GAAGAC GAAGAC(2/6)
BglII 680 AGATCT A^GATCT
BpmI 253 CTGGAG CTGGAG(16/14)
BpuAI 393 GAAGAC GAAGAC(2/6)
BsgI 358 GTGCAG GTGCAG(16/14)
BsiHKAI 706 GAGCAC G[AT]GC[AT]^C
BsmFI 667 GGGAC GGGAC(10/14)
BsoBI 826 CCCGAG C^[CT]CG[GA]G
BsrBI 445 GAGCGG CCGCTC(-3/-3)
BstXI 365 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 680 AGATCT [GA]^GATC[CT]
DraII 24 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 380 TGGCCA [CT]^GGCC[GA]
Eam1105I 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 24 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 184 GAATTC G^AATTC
HincII 231 GTCAAC GT[CT]^[GA]AC
HindII 231 GTCAAC GT[CT]^[GA]AC
MaeII 416 ACGT A^CGT
MaeIII 121 GTGAC ^GT[ACGT]AC
MflI 680 AGATCT [GA]^GATC[CT]
MluNI 380 TGGCCA TGG^CCA
MscI 380 TGGCCA TGG^CCA
MseI 53 TTAA T^TAA
MslI 771 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 588 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 250 CAGCTG CAG^CTG
ScaI 812 AGTACT AGT^ACT
Tru9I 53 TTAA T^TAA
Tsp45I 121 GTGAC ^GT[GC]AC
TspRI 709 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 588 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 680 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 184 GAATTC [GA]^AATT[CT]
451 AAATTC
AcyI 554 GGCGCC G[GA]^CG[CT]C
754 GGCGCC
AlwNI 94 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
589 CAGCCTCTG
ApoI 184 GAATTC [GA]^AATT[CT]
451 AAATTC
AvaII 597 GGACC G^G[AT]CC
668 GGACC
BbeI 554 GGCGCC GGCGC^C
754 GGCGCC
BsaHI 554 GGCGCC G[GA]^CG[CT]C
754 GGCGCC
DdeI 100 CTGAG C^T[ACGT]AG
469 CTAAG
HaeII 554 GGCGCC [GA]GCGC^[CT]
754 GGCGCC
Hin1I 554 GGCGCC G[GA]^CG[CT]C
754 GGCGCC
HinfI 229 GAGTC G^A[ACGT]TC
822 GACTC
KasI 554 GGCGCC G^GCGCC
754 GGCGCC
NarI 554 GGCGCC GG^CGCC
754 GGCGCC
PleI 822 GACTC GAGTC(4/5)
229 GAGTC
SfaNI 134 GCATC GCATC(5/9)
170 GCATC
SfoI 554 GGCGCC GGC^GCC
754 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DpnII EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TseI Tsp509I TthHB8I