Restriction map for
pFN21AE5635
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BfaI BfrI
BglI BlnI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 214 GACGTC GACGT^C
AccI 736 GTCTAC GT^[AC][GT]AC
AcsI 1102 AAATTC [GA]^AATT[CT]
AlwNI 1018 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 668 GGGCCC GGGCC^C
ApoI 1102 AAATTC [GA]^AATT[CT]
AspI 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 284 TGCGCA TGC^GCA
BalI 974 TGGCCA TGG^CCA
BbsI 193 GAAGAC GAAGAC(2/6)
BbvCI 639 CCTCAGC CCTCAGC(-5/-2)
BglII 45 AGATCT A^GATCT
BlpI 762 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 762 GCTCAGC GC^T[ACGT]AGC
BpuAI 193 GAAGAC GAAGAC(2/6)
BsaBI 282 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 752 GGTCTC GGTCTC(1/5)
BsiEI 98 CGGCCG CG[GA][CT]^CG
BsmI 204 GAATGC GAATGC(1/-1)
Bsp1407I 1202 TGTACA T^GTACA
BspMI 1160 ACCTGC ACCTGC(4/8)
BsrBI 462 GAGCGG CCGCTC(-3/-3)
BsrFI 1013 ACCGGC [GA]^CCGG[CT]
BsrGI 1202 TGTACA T^GTACA
BstAPI 1017 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 965 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 762 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1013 ACCGGC [GA]^CCGG[CT]
EagI 98 CGGCCG C^GGCCG
EclXI 98 CGGCCG C^GGCCG
Eco52I 98 CGGCCG C^GGCCG
EcoT22I 1264 ATGCAT ATGCA^T
FspI 284 TGCGCA TGC^GCA
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
HphI 845 TCACC GGTGA(8/7)
MamI 282 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 974 TGGCCA TGG^CCA
MscI 974 TGGCCA TGG^CCA
MseI 377 TTAA T^TAA
NsiI 1264 ATGCAT ATGCA^T
PflFI 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 618 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1478 CAGCTG CAG^CTG
SspBI 1202 TGTACA T^GTACA
TfiI 1212 GATTC G^A[AT]TC
Tru9I 377 TTAA T^TAA
Tth111I 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 618 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 214 GACGTC G[GA]^CG[CT]C
448 GACGCC
BsaAI 223 TACGTG [CT]AC^GT[GA]
1249 TACGTG
BsaHI 214 GACGTC G[GA]^CG[CT]C
448 GACGCC
BsrDI 306 CATTGC GCAATG(2/0)
1031 GCAATG
BsrI 1233 CCAGT ACTGG(1/-1)
1557 CCAGT
DsaI 158 CCGTGG C^C[GA][CT]GG
1292 CCACGG
EcoT14I 998 CCTTGG C^C[AT][AT]GG
1457 CCAAGG
Hin1I 214 GACGTC G[GA]^CG[CT]C
448 GACGCC
MslI 702 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1572 CACCAACATG
MspA1I 273 CAGCGG C[AC]G^C[GT]G
1478 CAGCTG
PpuMI 635 GGGACCT [GA]G^G[AT]CC[CT]
1195 AGGACCC
StyI 998 CCTTGG C^C[AT][AT]GG
1457 CCAAGG
TaqI 176 TCGA T^CGA
290 TCGA
Tsp509I 748 AATT ^AATT
1103 AATT
TthHB8I 176 TCGA T^CGA
290 TCGA
XcmI 239 CCAGCCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
323 CCACGCTGCGGGTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaI AvaII BanII
BbvI BclI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI TseI Tsp45I
TspRI XhoII XmaCI XmaI