Restriction map for
pFN21AE3020
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiHKAI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI DdeI DpnII DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI KpnI Ksp632I MaeI MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NdeII NheI
NlaIII NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 265 GACGTC GACGT^C
Asp700I 333 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 192 CCCGGG C^[CT]CG[GA]G
BbsI 337 GTCTTC GAAGAC(2/6)
BglI 28 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 91 GGGCAC G[AGT]GC[ACT]^C
BpuAI 337 GTCTTC GAAGAC(2/6)
BsgI 226 CTGCAC GTGCAG(16/14)
BsiWI 152 CGTACG C^GTACG
BsoBI 192 CCCGGG C^[CT]CG[GA]G
Bsp1286I 91 GGGCAC G[AGT]GC[ACT]^C
BsrBI 292 GAGCGG CCGCTC(-3/-3)
BsrFI 202 GCCGGC [GA]^CCGG[CT]
BssHII 351 GCGCGC G^CGCGC
Cfr10I 202 GCCGGC [GA]^CCGG[CT]
CpoI 7 CGGTCCG CG^G[AT]CCG
HgaI 257 GCGTC GACGC(5/10)
MboII 338 TCTTC GAAGA(8/7)
NaeI 202 GCCGGC GCC^GGC
NgoMIV 202 GCCGGC G^CCGGC
NotI 22 GCGGCCGC GC^GGCCGC
PstI 280 CTGCAG CTGCA^G
RsrII 7 CGGTCCG CG^G[AT]CCG
SmaI 192 CCCGGG CCC^GGG
XmaCI 192 CCCGGG C^CCGGG
XmaI 192 CCCGGG C^CCGGG
XmnI 333 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 75 GTAC GT^AC
153 GTAC
AluI 97 AGCT AG^CT
218 AGCT
BanI 54 GGCGCC G^G[CT][GA]CC
205 GGCGCC
BbeI 54 GGCGCC GGCGC^C
205 GGCGCC
BsiEI 23 CGGCCG CG[GA][CT]^CG
306 CGGCCG
BspMI 278 ACCTGC ACCTGC(4/8)
143 GCAGGT
BsrI 18 ACTGG ACTGG(1/-1)
321 CCAGT
DraII 145 AGGTCCC [GA]G^G[ACGT]CC[CT]
313 GGGACCT
DsaI 119 CCGCGG C^C[GA][CT]GG
309 CCGCGG
EaeI 23 CGGCCG [CT]^GGCC[GA]
306 CGGCCG
EagI 23 CGGCCG C^GGCCG
306 CGGCCG
EclXI 23 CGGCCG C^GGCCG
306 CGGCCG
Eco52I 23 CGGCCG C^GGCCG
306 CGGCCG
EcoO109I 145 AGGTCCC [GA]G^G[ACGT]CC[CT]
313 GGGACCT
KasI 54 GGCGCC G^GCGCC
205 GGCGCC
KspI 119 CCGCGG CCGC^GG
309 CCGCGG
MaeII 266 ACGT A^CGT
335 ACGT
NarI 54 GGCGCC GG^CGCC
205 GGCGCC
PpuMI 145 AGGTCCC [GA]G^G[AT]CC[CT]
313 GGGACCT
RsaI 75 GTAC GT^AC
153 GTAC
SacII 119 CCGCGG CCGC^GG
309 CCGCGG
SfcI 174 CTACAG C^T[GA][CT]AG
280 CTGCAG
SfoI 54 GGCGCC GGC^GCC
205 GGCGCC
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
322 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AcyI AvaII BbvI
BcnI BsaHI BsaJI BsiYI BslI
BsmFI BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI