Restriction map for
pFN21AE1723
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 115 CGCG CG^CG
AflIII 114 ACGCGT A^C[GA][CT]GT
AlwI 60 GATCC GGATC(4/5)
AocI 578 CCTCAGG CC^T[ACGT]AGG
AvaII 377 GGACC G^G[AT]CC
BalI 335 TGGCCA TGG^CCA
BanI 417 GGTGCC G^G[CT][GA]CC
BbsI 348 GAAGAC GAAGAC(2/6)
BclI 155 TGATCA T^GATCA
BpuAI 348 GAAGAC GAAGAC(2/6)
BsaWI 206 ACCGGA [AT]^CCGG[AT]
BsmFI 376 GGGAC GGGAC(10/14)
BspMI 14 ACCTGC ACCTGC(4/8)
BsrDI 684 GCAATG GCAATG(2/0)
BstUI 115 CGCG CG^CG
BstYI 59 AGATCC [GA]^GATC[CT]
Bsu36I 578 CCTCAGG CC^T[ACGT]AGG
CfoI 223 GCGC GCG^C
DraIII 544 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 113 GACGCGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 578 CCTCAGG CC^T[ACGT]AGG
EcoNI 380 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 229 CCCGC CCCGC(4/6)
FbaI 155 TGATCA T^GATCA
HaeII 222 GGCGCT [GA]GCGC^[CT]
HhaI 223 GCGC GCG^C
HinP1I 223 GCGC G^CGC
MaeII 248 ACGT A^CGT
MboII 348 GAAGA GAAGA(8/7)
MflI 59 AGATCC [GA]^GATC[CT]
MluI 114 ACGCGT A^CGCGT
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
MseI 11 TTAA T^TAA
MspA1I 421 CCGCTG C[AC]G^C[GT]G
MvnI 115 CGCG CG^CG
PpuMI 376 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 413 ACCTGGT A^CC[AT]GGT
SfaNI 284 GCATC GCATC(5/9)
SfcI 180 CTACAG C^T[GA][CT]AG
SfiI 252 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 50 CCCGGG CCC^GGG
StuI 575 AGGCCT AGG^CCT
Tru9I 11 TTAA T^TAA
Tsp509I 357 AATT ^AATT
XcmI 404 CCATCCTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 59 AGATCC [GA]^GATC[CT]
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 141 GTAC GT^AC
540 GTAC
AlwNI 189 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
676 CAGATTCTG
AvaI 50 CCCGGG C^[CT]CG[GA]G
73 CTCGGG
BglI 253 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
588 GCCTGCCGGGC
BsgI 195 CTGCAC GTGCAG(16/14)
365 CTGCAC
BsmAI 111 GAGAC GTCTC(1/5)
245 GAGAC
BsmBI 111 GAGACG CGTCTC(1/5)
245 GAGACG
BsoBI 50 CCCGGG C^[CT]CG[GA]G
73 CTCGGG
BsrI 369 ACTGG ACTGG(1/-1)
619 CCAGT
BstF5I 405 CATCC GGATG(2/0)
372 GGATG
DpnII 60 GATC ^GATC
156 GATC
DraII 260 AGGCCCT [GA]G^G[ACGT]CC[CT]
376 GGGACCT
EaeI 335 TGGCCA [CT]^GGCC[GA]
425 TGGCCG
EcoO109I 260 AGGCCCT [GA]G^G[ACGT]CC[CT]
376 GGGACCT
EcoT14I 215 CCATGG C^C[AT][AT]GG
332 CCATGG
FokI 405 CATCC GGATG(9/13)
372 GGATG
HgaI 113 GACGC GACGC(5/10)
116 GCGTC
HinfI 69 GATTC G^A[ACGT]TC
678 GATTC
MboI 60 GATC ^GATC
156 GATC
NcoI 215 CCATGG C^CATGG
332 CCATGG
NdeII 60 GATC ^GATC
156 GATC
RsaI 141 GTAC GT^AC
540 GTAC
Sau3AI 60 GATC ^GATC
156 GATC
StyI 215 CCATGG C^C[AT][AT]GG
332 CCATGG
TaqI 287 TCGA T^CGA
515 TCGA
TfiI 69 GATTC G^A[AT]TC
678 GATTC
TthHB8I 287 TCGA T^CGA
515 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstAPI BstNI
Cac8I Cfr13I DdeI DsaI EcoRII
Fnu4HI HaeIII HapII HpaII HphI
ItaI MnlI MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI TspRI