Restriction map for
pFN21AE9569
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
Hin1I HindIII HpaI KasI KpnI
MaeI MfeI MflI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SbfI ScaI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 652 GTCGAC GT^[AC][GT]AC
Alw44I 301 GTGCAC G^TGCAC
AlwI 711 GATCC GGATC(4/5)
ApaLI 301 GTGCAC G^TGCAC
AspHI 301 GTGCAC G[AT]GC[AT]^C
AviII 407 TGCGCA TGC^GCA
BbsI 577 GTCTTC GAAGAC(2/6)
BpuAI 577 GTCTTC GAAGAC(2/6)
BsaBI 711 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 301 GTGCAC G[AT]GC[AT]^C
BsmFI 1058 GTCCC GGGAC(10/14)
BspMI 587 ACCTGC ACCTGC(4/8)
BsrBI 793 CCGCTC CCGCTC(-3/-3)
BssSI 109 CTCGTG CACGAG(-5/-1)
BstAPI 911 GCACGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 481 CTGAG C^T[ACGT]AG
DpnII 711 GATC ^GATC
EcoT14I 557 CCATGG C^C[AT][AT]GG
EcoT22I 1083 ATGCAT ATGCA^T
FspI 407 TGCGCA TGC^GCA
HgaI 857 GCGTC GACGC(5/10)
HinfI 1012 GAGTC G^A[ACGT]TC
KspI 665 CCGCGG CCGC^GG
MaeII 5 ACGT A^CGT
MaeIII 700 GTGAC ^GT[ACGT]AC
MamI 711 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 711 GATC ^GATC
MseI 194 TTAA T^TAA
MslI 129 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 557 CCATGG C^CATGG
NdeII 711 GATC ^GATC
NsiI 1083 ATGCAT ATGCA^T
PaeR7I 1090 CTCGAG C^TCGAG
PleI 1012 GAGTC GAGTC(4/5)
PvuII 502 CAGCTG CAG^CTG
SacII 665 CCGCGG CCGC^GG
SalI 652 GTCGAC G^TCGAC
Sau3AI 711 GATC ^GATC
SexAI 833 ACCTGGT A^CC[AT]GGT
SfiI 925 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1090 CTCGAG C^T[CT][GA]AG
StyI 557 CCATGG C^C[AT][AT]GG
Tru9I 194 TTAA T^TAA
Tsp45I 700 GTGAC ^GT[GC]AC
TspRI 990 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 251 CCATCTACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1090 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AscI 644 GGCGCGCC GG^CGCGCC
683 GGCGCGCC
BalI 374 TGGCCA TGG^CCA
506 TGGCCA
BglI 38 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
926 GCCTGCTGGGC
BsaI 837 GGTCTC GGTCTC(1/5)
882 GGTCTC
BsgI 391 CTGCAC GTGCAG(16/14)
567 CTGCAC
BsmBI 6 CGTCTC CGTCTC(1/5)
354 CGTCTC
BsrI 495 CCAGT ACTGG(1/-1)
786 CCAGT
HaeII 538 AGCGCC [GA]GCGC^[CT]
1069 GGCGCT
HincII 652 GTCGAC GT[CT]^[GA]AC
859 GTCAAC
HindII 652 GTCGAC GT[CT]^[GA]AC
859 GTCAAC
HphI 699 GGTGA GGTGA(8/7)
320 TCACC
MluNI 374 TGGCCA TGG^CCA
506 TGGCCA
MscI 374 TGGCCA TGG^CCA
506 TGGCCA
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
SapI Sau96I ScrFI SfaNI TaqI
TseI TthHB8I