Restriction map for
pFN21AE5275
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# NO CUT ENZYMES ( 156 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 18 GACGTC GACGT^C
AccII 124 CGCG CG^CG
AfaI 138 GTAC GT^AC
AocI 354 CCTCAGG CC^T[ACGT]AGG
AspI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 423 GGACC G^G[AT]CC
AviII 608 TGCGCA TGC^GCA
BanI 388 GGCGCC G^G[CT][GA]CC
BanII 340 GAGCCC G[GA]GC[CT]^C
BbeI 388 GGCGCC GGCGC^C
BbsI 364 GTCTTC GAAGAC(2/6)
BglII 488 AGATCT A^GATCT
BmyI 340 GAGCCC G[AGT]GC[ACT]^C
BpmI 337 CTGGAG CTGGAG(16/14)
BpuAI 364 GTCTTC GAAGAC(2/6)
BseRI 352 CTCCTC GAGGAG(10/8)
BsmAI 598 GAGAC GTCTC(1/5)
BsmFI 21 GTCCC GGGAC(10/14)
Bsp1286I 340 GAGCCC G[AGT]GC[ACT]^C
BsrBI 624 CCGCTC CCGCTC(-3/-3)
BstUI 124 CGCG CG^CG
BstXI 371 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 AGATCT [GA]^GATC[CT]
Bsu36I 354 CCTCAGG CC^T[ACGT]AGG
Cfr13I 423 GGACC G^G[ACGT]CC
DpnII 489 GATC ^GATC
DraII 422 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 397 GACAGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 354 CCTCAGG CC^T[ACGT]AGG
EcoNI 92 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 422 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 608 TGCGCA TGC^GCA
HaeII 388 GGCGCC [GA]GCGC^[CT]
HaeIII 40 GGCC GG^CC
HinfI 244 GAGTC G^A[ACGT]TC
HphI 270 GGTGA GGTGA(8/7)
KasI 388 GGCGCC G^GCGCC
MboI 489 GATC ^GATC
MflI 488 AGATCT [GA]^GATC[CT]
MslI 529 CACAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 124 CGCG CG^CG
NarI 388 GGCGCC GG^CGCC
NdeI 149 CATATG CA^TATG
NdeII 489 GATC ^GATC
PflFI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 244 GAGTC GAGTC(4/5)
PpuMI 422 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 138 GTAC GT^AC
Sau3AI 489 GATC ^GATC
Sau96I 423 GGACC G^G[ACGT]CC
SfoI 388 GGCGCC GGC^GCC
SmlI 547 CTCAAG C^T[CT][GA]AG
TaqI 596 TCGA T^CGA
Tth111I 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 596 TCGA T^CGA
XhoII 488 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
BcnI 14 CCCGG CC^[GC]GG
476 CCCGG
BfaI 295 CTAG C^TAG
427 CTAG
BsaHI 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
BsaJI 392 CCTCGG C^C[ACGT][ACGT]GG
475 CCCCGG
BsgI 346 GTGCAG GTGCAG(16/14)
616 GTGCAG
BsrI 481 ACTGG ACTGG(1/-1)
327 CCAGT
BstF5I 324 CATCC GGATG(2/0)
438 CATCC
DdeI 240 CTCAG C^T[ACGT]AG
355 CTCAG
FokI 324 CATCC GGATG(9/13)
438 CATCC
HapII 15 CCGG C^CGG
477 CCGG
HgaI 538 GACGC GACGC(5/10)
362 GCGTC
Hin1I 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
HpaII 15 CCGG C^CGG
477 CCGG
MaeI 295 CTAG C^TAG
427 CTAG
MaeII 19 ACGT A^CGT
404 ACGT
MspA1I 142 CAGCTG C[AC]G^C[GT]G
639 CAGCTG
MspI 15 CCGG C^CGG
477 CCGG
NciI 14 CCCGG CC^[GC]GG
476 CCCGG
NlaIII 332 CATG CATG^
585 CATG
PstI 5 CTGCAG CTGCA^G
211 CTGCAG
PvuII 142 CAGCTG CAG^CTG
639 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BssKI BstNI Cac8I CfoI EcoRII
Fnu4HI HhaI HinP1I ItaI MboII
MnlI MvaI MwoI NlaIV ScrFI
SfaNI SfcI TseI TspRI