##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AfaI          105  GTAC                 GT^AC
AlwNI          64  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         265  GAGCTC               G[AT]GC[AT]^C
AvaII         239  GGTCC                G^G[AT]CC
AvrII         187  CCTAGG               C^CTAGG
BanI           76  GGTGCC               G^G[CT][GA]CC
BfaI          188  CTAG                 C^TAG
BlnI          187  CCTAGG               C^CTAGG
BlpI          267  GCTCAGC              GC^T[ACGT]AGC
BpmI           94  CTGGAG               CTGGAG(16/14)
Bpu1102I      267  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       265  GAGCTC               G[AT]GC[AT]^C
BspMI          43  ACCTGC               ACCTGC(4/8)
BstXI          41  CCACCTGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         267  GCTCAGC              GC^T[ACGT]AGC
DdeI          268  CTCAG                C^T[ACGT]AG
DpnII         154  GATC                 ^GATC
DraII         238  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoO109I      238  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       187  CCTAGG               C^C[AT][AT]GG
MaeI          188  CTAG                 C^TAG
MaeIII         57  GTTAC                ^GT[ACGT]AC
MboI          154  GATC                 ^GATC
NdeII         154  GATC                 ^GATC
PpuMI         238  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         149  CAGCTG               CAG^CTG
RsaI          105  GTAC                 GT^AC
SacI          265  GAGCTC               GAGCT^C
Sau3AI        154  GATC                 ^GATC
SfcI           61  CTACAG               C^T[GA][CT]AG
SphI          246  GCATGC               GCATG^C
StuI          184  AGGCCT               AGG^CCT
StyI          187  CCTAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI          150  AGCT                 AG^CT
              266  AGCT                
BanII          86  GAGCCC               G[GA]GC[CT]^C
              265  GAGCTC              
Cfr13I        138  GGCCC                G^G[ACGT]CC
              239  GGTCC               
FauI          243  CCCGC                CCCGC(4/6)
              273  CCCGC               
MslI           42  CACCTGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              168  CATCAACATG          
MspA1I        149  CAGCTG               C[AC]G^C[GT]G
              274  CCGCTG              
NlaIII        174  CATG                 CATG^
              247  CATG                
NspI          173  ACATGC               [GA]CATG^[CT]
              246  GCATGC              
Sau96I        138  GGCCC                G^G[ACGT]CC
              239  GGTCC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       EcoRII      Fnu4HI      HaeIII      
ItaI        MnlI        MvaI        MwoI        NlaIV       
ScrFI       TseI        TspRI