Restriction map for
pFN21AE1702
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 415 GACGTC GACGT^C
AccII 586 CGCG CG^CG
AcyI 415 GACGTC G[GA]^CG[CT]C
AfaI 582 GTAC GT^AC
Alw44I 65 GTGCAC G^TGCAC
Aor51HI 177 AGCGCT AGC^GCT
ApaI 99 GGGCCC GGGCC^C
ApaLI 65 GTGCAC G^TGCAC
AspHI 65 GTGCAC G[AT]GC[AT]^C
BanI 493 GGTGCC G^G[CT][GA]CC
BcnI 528 CCGGG CC^[GC]GG
BglI 496 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 415 GACGTC G[GA]^CG[CT]C
BseRI 223 GAGGAG GAGGAG(10/8)
BsgI 79 GTGCAG GTGCAG(16/14)
BsiHKAI 65 GTGCAC G[AT]GC[AT]^C
BsmFI 389 GTCCC GGGAC(10/14)
BspMI 9 ACCTGC ACCTGC(4/8)
BsrFI 501 GCCGGC [GA]^CCGG[CT]
BsrI 459 ACTGG ACTGG(1/-1)
BstAPI 45 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 255 CATCC GGATG(2/0)
BstUI 586 CGCG CG^CG
Cfr10I 501 GCCGGC [GA]^CCGG[CT]
Eco47III 177 AGCGCT AGC^GCT
EcoNI 339 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 395 CCTTGG C^C[AT][AT]GG
FokI 255 CATCC GGATG(9/13)
Hin1I 415 GACGTC G[GA]^CG[CT]C
MaeII 416 ACGT A^CGT
MvnI 586 CGCG CG^CG
NaeI 501 GCCGGC GCC^GGC
NciI 528 CCGGG CC^[GC]GG
NgoMIV 501 GCCGGC G^CCGGC
PflMI 434 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 582 GTAC GT^AC
SbfI 354 CCTGCAGG CCTGCA^GG
SfaNI 254 GCATC GCATC(5/9)
Sse8387I 354 CCTGCAGG CCTGCA^GG
StyI 395 CCTTGG C^C[AT][AT]GG
Van91I 434 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 26 CCATGAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 585 CCGC CCGC(-3/-1)
329 GCGG
AlwI 159 GATCC GGATC(4/5)
371 GGATC
AvaI 220 CCCGAG C^[CT]CG[GA]G
379 CCCGAG
BpmI 181 CTCCAG CTGGAG(16/14)
571 CTGGAG
BsoBI 220 CCCGAG C^[CT]CG[GA]G
379 CCCGAG
BstXI 89 CCACCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
162 CCAGGGCGATGG
BstYI 158 AGATCC [GA]^GATC[CT]
371 GGATCT
DdeI 141 CTTAG C^T[ACGT]AG
271 CTGAG
DpnII 159 GATC ^GATC
372 GATC
HaeII 177 AGCGCT [GA]GCGC^[CT]
524 AGCGCC
HapII 502 CCGG C^CGG
528 CCGG
HpaII 502 CCGG C^CGG
528 CCGG
MboI 159 GATC ^GATC
372 GATC
MboII 156 GAAGA GAAGA(8/7)
454 GAAGA
MflI 158 AGATCC [GA]^GATC[CT]
371 GGATCT
MspI 502 CCGG C^CGG
528 CCGG
NdeII 159 GATC ^GATC
372 GATC
NlaIII 27 CATG CATG^
498 CATG
PpuMI 387 GGGTCCC [GA]G^G[AT]CC[CT]
539 GGGTCCT
Sau3AI 159 GATC ^GATC
372 GATC
SmlI 133 CTCAAG C^T[CT][GA]AG
312 CTTGAG
XhoII 158 AGATCC [GA]^GATC[CT]
371 GGATCT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwNI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DraII EcoO109I EcoRII Fnu4HI HaeIII
HhaI HinP1I ItaI MnlI MspA1I
MvaI MwoI NlaIV PstI PvuII
Sau96I ScrFI SfcI TseI TspRI