Restriction map for
pFN21AE3085
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 296 GATCC GGATC(4/5)
AlwNI 502 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 171 CCTCAGG CC^T[ACGT]AGG
ApaI 57 GGGCCC GGGCC^C
AspHI 316 GTGCTC G[AT]GC[AT]^C
BanII 57 GGGCCC G[GA]GC[CT]^C
BbsI 646 GTCTTC GAAGAC(2/6)
BciVI 231 GTATCC GTATCC(6/5)
BfaI 25 CTAG C^TAG
BpuAI 646 GTCTTC GAAGAC(2/6)
BsgI 352 CTGCAC GTGCAG(16/14)
BsiHKAI 316 GTGCTC G[AT]GC[AT]^C
BsmFI 199 GGGAC GGGAC(10/14)
BspMI 640 GCAGGT ACCTGC(4/8)
BsrI 61 CCAGT ACTGG(1/-1)
BstYI 295 AGATCC [GA]^GATC[CT]
Bsu36I 171 CCTCAGG CC^T[ACGT]AGG
CfoI 638 GCGC GCG^C
CpoI 611 CGGACCG CG^G[AT]CCG
DpnII 296 GATC ^GATC
Eco81I 171 CCTCAGG CC^T[ACGT]AGG
HhaI 638 GCGC GCG^C
HinP1I 638 GCGC G^CGC
MaeI 25 CTAG C^TAG
MaeII 518 ACGT A^CGT
MboI 296 GATC ^GATC
MflI 295 AGATCC [GA]^GATC[CT]
MspA1I 627 CAGCTG C[AC]G^C[GT]G
NdeII 296 GATC ^GATC
NheI 24 GCTAGC G^CTAGC
PaeR7I 548 CTCGAG C^TCGAG
PpuMI 503 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 627 CAGCTG CAG^CTG
RsrII 611 CGGACCG CG^G[AT]CCG
Sau3AI 296 GATC ^GATC
SexAI 356 ACCTGGT A^CC[AT]GGT
SmaI 689 CCCGGG CCC^GGG
SmlI 548 CTCGAG C^T[CT][GA]AG
StuI 438 AGGCCT AGG^CCT
TaqI 549 TCGA T^CGA
TfiI 467 GAATC G^A[AT]TC
Tsp509I 684 AATT ^AATT
TspRI 314 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 549 TCGA T^CGA
XcmI 530 CCATGGGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 548 CTCGAG C^TCGAG
XhoII 295 AGATCC [GA]^GATC[CT]
XmaCI 689 CCCGGG C^CCGGG
XmaI 689 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 548 CTCGAG C^[CT]CG[GA]G
689 CCCGGG
AvaII 504 GGTCC G^G[AT]CC
612 GGACC
BmyI 57 GGGCCC G[AGT]GC[ACT]^C
316 GTGCTC
BpmI 41 CTCCAG CTGGAG(16/14)
479 CTGGAG
BsoBI 548 CTCGAG C^[CT]CG[GA]G
689 CCCGGG
Bsp1286I 57 GGGCCC G[AGT]GC[ACT]^C
316 GTGCTC
BsrFI 72 GCCGGC [GA]^CCGG[CT]
188 GCCGGC
BstF5I 384 GGATG GGATG(2/0)
474 GGATG
BstXI 450 CCAAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
471 CCAGGATGCTGG
Cfr10I 72 GCCGGC [GA]^CCGG[CT]
188 GCCGGC
FokI 384 GGATG GGATG(9/13)
474 GGATG
MaeIII 397 GTGAC ^GT[ACGT]AC
520 GTGAC
MboII 333 GAAGA GAAGA(8/7)
647 TCTTC
MseI 368 TTAA T^TAA
707 TTAA
NaeI 72 GCCGGC GCC^GGC
188 GCCGGC
NcoI 134 CCATGG C^CATGG
530 CCATGG
NgoMIV 72 GCCGGC G^CCGGC
188 GCCGGC
PstI 163 CTGCAG CTGCA^G
193 CTGCAG
SfaNI 265 GATGC GCATC(5/9)
475 GATGC
Tru9I 368 TTAA T^TAA
707 TTAA
Tsp45I 397 GTGAC ^GT[GC]AC
520 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BcnI BsaJI BsiYI
BslI BsmAI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI SfcI
StyI TseI