Restriction map for
pFN21AE2725
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII Alw44I AocI ApaLI ApoI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MaeI MamI MfeI
MroI MunI NdeI NheI NruI
NsiI NspI NspV PacI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SapI SbfI ScaI SexAI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
XbaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 979 GTCGAC GT^[AC][GT]AC
AgeI 105 ACCGGT A^CCGGT
AhdI 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 858 GATCC GGATC(4/5)
Aor51HI 667 AGCGCT AGC^GCT
AscI 592 GGCGCGCC GG^CGCGCC
AspEI 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 180 GAGCAC G[AT]GC[AT]^C
AvaII 881 GGACC G^G[AT]CC
BalI 50 TGGCCA TGG^CCA
BbsI 265 GAAGAC GAAGAC(2/6)
BciVI 1153 GGATAC GTATCC(6/5)
BglII 998 AGATCT A^GATCT
BpuAI 265 GAAGAC GAAGAC(2/6)
BsaI 112 GAGACC GGTCTC(1/5)
BsaWI 105 ACCGGT [AT]^CCGG[AT]
BsiHKAI 180 GAGCAC G[AT]GC[AT]^C
BsmBI 142 CGTCTC CGTCTC(1/5)
BspMI 32 ACCTGC ACCTGC(4/8)
BsrBI 522 GAGCGG CCGCTC(-3/-3)
BsrDI 16 GCAATG GCAATG(2/0)
BstAPI 182 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 798 GGTGACC G^GT[ACGT]ACC
BstF5I 278 GGATG GGATG(2/0)
BstPI 798 GGTGACC G^GT[ACGT]ACC
BstXI 41 CCAGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 998 AGATCT [GA]^GATC[CT]
DdeI 1121 CTGAG C^T[ACGT]AG
DraII 471 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 183 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 873 GAAGAG CTCTTC(1/4)
Eco47III 667 AGCGCT AGC^GCT
EcoNI 987 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 471 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 798 GGTGACC G^GT[ACGT]ACC
FokI 278 GGATG GGATG(9/13)
HincII 979 GTCGAC GT[CT]^[GA]AC
HindII 979 GTCGAC GT[CT]^[GA]AC
HinfI 949 GAGTC G^A[ACGT]TC
Ksp632I 873 GAAGAG CTCTTC(1/4)
MaeII 941 ACGT A^CGT
MaeIII 799 GTGAC ^GT[ACGT]AC
MflI 998 AGATCT [GA]^GATC[CT]
MluI 1025 ACGCGT A^CGCGT
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MseI 65 TTAA T^TAA
NcoI 53 CCATGG C^CATGG
PaeR7I 1080 CTCGAG C^TCGAG
PflMI 1160 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 105 ACCGGT A^CCGGT
PleI 949 GAGTC GAGTC(4/5)
PstI 970 CTGCAG CTGCA^G
SalI 979 GTCGAC G^TCGAC
SfcI 970 CTGCAG C^T[GA][CT]AG
SgrAI 753 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 231 CCCGGG CCC^GGG
Tru9I 65 TTAA T^TAA
Tsp45I 799 GTGAC ^GT[GC]AC
Van91I 1160 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1080 CTCGAG C^TCGAG
XhoII 998 AGATCT [GA]^GATC[CT]
XmaCI 231 CCCGGG C^CCGGG
XmaI 231 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 1029 GTAC GT^AC
1186 GTAC
AflIII 939 ACACGT A^C[GA][CT]GT
1025 ACGCGT
ApaI 471 GGGCCC GGGCC^C
531 GGGCCC
AspI 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
801 GACCATGTC
BseRI 463 GAGGAG GAGGAG(10/8)
850 GAGGAG
BsmFI 1022 GGGAC GGGAC(10/14)
44 GTCCC
BssHII 394 GCGCGC G^CGCGC
593 GCGCGC
EcoT14I 53 CCATGG C^C[AT][AT]GG
446 CCTTGG
HgaI 334 GACGC GACGC(5/10)
1024 GACGC
MslI 171 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
804 CATGTCTGTG
NotI 508 GCGGCCGC GC^GGCCGC
616 GCGGCCGC
PflFI 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
801 GACCATGTC
RsaI 1029 GTAC GT^AC
1186 GTAC
SfiI 414 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
618 GGCCGCCTCGGCC
SmlI 988 CTCAAG C^T[CT][GA]AG
1080 CTCGAG
StyI 53 CCATGG C^C[AT][AT]GG
446 CCTTGG
Tth111I 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
801 GACCATGTC
XcmI 38 CCACCAGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1127 CCACTCGCAAGTTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiEI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI KspI MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PvuII SacII Sau3AI
Sau96I ScrFI SfaNI SfoI TaqI
TseI TspRI TthHB8I