Restriction map for
pFN21AE9638
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AviII BamHI
BbeI BbvCI BciVI BfrI BglI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I FseI FspI HpaI
KasI KpnI KspI MluI NarI
NotI NspV PaeR7I PinAI PmeI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 359 CGCG CG^CG
AclI 685 AACGTT AA^CGTT
AcyI 3353 GGCGTC G[GA]^CG[CT]C
AflIII 5078 ACATGT A^C[GA][CT]GT
AhdI 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5262 TGGCCA TGG^CCA
BbrPI 3470 CACGTG CAC^GTG
BclI 5235 TGATCA T^GATCA
BglII 3504 AGATCT A^GATCT
BlpI 3478 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3478 GCTGAGC GC^T[ACGT]AGC
BsaAI 3470 CACGTG [CT]AC^GT[GA]
BsaBI 4381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3353 GGCGTC G[GA]^CG[CT]C
BspHI 5160 TCATGA T^CATGA
BspLU11I 5078 ACATGT A^CATGT
BstEII 1696 GGTGACC G^GT[ACGT]ACC
BstPI 1696 GGTGACC G^GT[ACGT]ACC
BstUI 359 CGCG CG^CG
CelII 3478 GCTGAGC GC^T[ACGT]AGC
DraI 1407 TTTAAA TTT^AAA
DrdI 2638 GACACAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3378 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1696 GGTGACC G^GT[ACGT]ACC
FbaI 5235 TGATCA T^GATCA
HaeII 3512 AGCGCC [GA]GCGC^[CT]
Hin1I 3353 GGCGTC G[GA]^CG[CT]C
HindIII 80 AAGCTT A^AGCTT
MamI 4381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1089 CAATTG C^AATTG
MluNI 5262 TGGCCA TGG^CCA
MscI 5262 TGGCCA TGG^CCA
MunI 1089 CAATTG C^AATTG
MvnI 359 CGCG CG^CG
NaeI 1212 GCCGGC GCC^GGC
NgoMIV 1212 GCCGGC G^CCGGC
NheI 1482 GCTAGC G^CTAGC
NruI 358 TCGCGA TCG^CGA
NspI 5078 ACATGT [GA]CATG^[CT]
PacI 3847 TTAATTAA TTAAT^TAA
PflFI 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3470 CACGTG CAC^GTG
PmlI 3470 CACGTG CAC^GTG
PshAI 5225 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 685 AACGTT AA^CGTT
PstI 5345 CTGCAG CTGCA^G
RcaI 5160 TCATGA T^CATGA
SacI 1963 GAGCTC GAGCT^C
SmlI 4679 CTCAAG C^T[CT][GA]AG
Tth111I 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 1732 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2140 GAACAGATTC
AspHI 1963 GAGCTC G[AT]GC[AT]^C
4570 GAGCAC
AvrII 1536 CCTAGG C^CTAGG
4096 CCTAGG
BcgI 3359 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
660 GCAGACTCGTCG
BlnI 1536 CCTAGG C^CTAGG
4096 CCTAGG
BsiHKAI 1963 GAGCTC G[AT]GC[AT]^C
4570 GAGCAC
BsmI 1369 GAATGC GAATGC(1/-1)
4101 GCATTC
BsrFI 1212 GCCGGC [GA]^CCGG[CT]
3891 GCCGGT
BssSI 566 CTCGTG CACGAG(-5/-1)
3379 CTCGTG
BstAPI 2285 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3469 GCACGTGATGC
BstXI 1590 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5016 CCACCATTATGG
Cfr10I 1212 GCCGGC [GA]^CCGG[CT]
3891 GCCGGT
EaeI 2838 TGGCCG [CT]^GGCC[GA]
5262 TGGCCA
EcoRI 1950 GAATTC G^AATTC
3215 GAATTC
EcoT22I 53 ATGCAT ATGCA^T
4846 ATGCAT
FauI 5245 CCCGC CCCGC(4/6)
389 GCGGG
HincII 2397 GTTGAC GT[CT]^[GA]AC
3027 GTCAAC
HindII 2397 GTTGAC GT[CT]^[GA]AC
3027 GTCAAC
NsiI 53 ATGCAT ATGCA^T
4846 ATGCAT
PflMI 133 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2502 CCAGCGTCTGG
SfcI 3928 CTACAG C^T[GA][CT]AG
5345 CTGCAG
Van91I 133 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2502 CCAGCGTCTGG
XmnI 1732 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2140 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII