Restriction map for
pFN21AE4113
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI Ksp632I
MamI MfeI MluI MroI MslI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI SalI SapI SbfI
ScaI SexAI SfcI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1055 GGTACC G^GTACC
AcyI 64 GGCGCC G[GA]^CG[CT]C
AflIII 428 ACATGT A^C[GA][CT]GT
Alw44I 253 GTGCAC G^TGCAC
Aor51HI 614 AGCGCT AGC^GCT
ApaI 268 GGGCCC GGGCC^C
ApaLI 253 GTGCAC G^TGCAC
Asp718I 1055 GGTACC G^GTACC
BalI 470 TGGCCA TGG^CCA
BamHI 1225 GGATCC G^GATCC
BbeI 64 GGCGCC GGCGC^C
BsaHI 64 GGCGCC G[GA]^CG[CT]C
BsgI 1354 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
Bsp1407I 431 TGTACA T^GTACA
BspDI 445 ATCGAT AT^CGAT
BspLU11I 428 ACATGT A^CATGT
BsrGI 431 TGTACA T^GTACA
BstAPI 786 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 445 ATCGAT AT^CGAT
CpoI 658 CGGTCCG CG^G[AT]CCG
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco47III 614 AGCGCT AGC^GCT
Eco52I 35 CGGCCG C^GGCCG
EcoRI 851 GAATTC G^AATTC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
HincII 526 GTCAAC GT[CT]^[GA]AC
HindII 526 GTCAAC GT[CT]^[GA]AC
HphI 800 TCACC GGTGA(8/7)
KasI 64 GGCGCC G^GCGCC
KpnI 1055 GGTACC GGTAC^C
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
MseI 421 TTAA T^TAA
NarI 64 GGCGCC GG^CGCC
NotI 34 GCGGCCGC GC^GGCCGC
PleI 909 GACTC GAGTC(4/5)
PshAI 1410 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 658 CGGTCCG CG^G[AT]CCG
SfiI 642 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 64 GGCGCC GGC^GCC
SgrAI 60 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 273 CCCGGG CCC^GGG
SmlI 1171 CTCAAG C^T[CT][GA]AG
SspBI 431 TGTACA T^GTACA
TfiI 1019 GATTC G^A[AT]TC
Tru9I 421 TTAA T^TAA
Tsp45I 706 GTCAC ^GT[GC]AC
XmaCI 273 CCCGGG C^CCGGG
XmaI 273 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 377 AAATTC [GA]^AATT[CT]
851 GAATTC
AlwNI 688 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
787 CAGGGGCTG
ApoI 377 AAATTC [GA]^AATT[CT]
851 GAATTC
BfaI 46 CTAG C^TAG
868 CTAG
BglI 643 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1121 GCCTGTATGGC
BsaI 353 GAGACC GGTCTC(1/5)
919 GAGACC
BstYI 1225 GGATCC [GA]^GATC[CT]
1430 AGATCC
HinfI 909 GACTC G^A[ACGT]TC
1019 GATTC
MaeI 46 CTAG C^TAG
868 CTAG
MaeIII 706 GTCAC ^GT[ACGT]AC
1232 GTAAC
MflI 1225 GGATCC [GA]^GATC[CT]
1430 AGATCC
NaeI 61 GCCGGC GCC^GGC
584 GCCGGC
NgoMIV 61 GCCGGC G^CCGGC
584 GCCGGC
NspI 428 ACATGT [GA]CATG^[CT]
819 GCATGT
SfaNI 929 GCATC GCATC(5/9)
1076 GCATC
TaqI 446 TCGA T^CGA
746 TCGA
Tsp509I 378 AATT ^AATT
852 AATT
TthHB8I 446 TCGA T^CGA
746 TCGA
XcmI 728 CCAAAGAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1295 CCAGTGCCGTCCTGG
XhoII 1225 GGATCC [GA]^GATC[CT]
1430 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI KspI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI SacI SacII
Sau3AI Sau96I ScrFI TseI TspRI