Restriction map for
pFN21AE6650
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# NO CUT ENZYMES ( 149 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BanI
BbeI BbsI BcgI BciVI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
HindIII HpaI KasI KpnI Ksp632I
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp509I
TspRI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 682 GACGTC GACGT^C
Aor51HI 36 AGCGCT AGC^GCT
ApaI 618 GGGCCC GGGCC^C
AspHI 550 GAGCTC G[AT]GC[AT]^C
AviII 158 TGCGCA TGC^GCA
BbrPI 472 CACGTG CAC^GTG
BbvCI 354 CCTCAGC CCTCAGC(-5/-2)
BclI 599 TGATCA T^GATCA
BglI 167 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 591 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 591 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 550 GAGCTC G[AT]GC[AT]^C
BsrBI 213 GAGCGG CCGCTC(-3/-3)
BsrI 438 ACTGG ACTGG(1/-1)
BstEII 582 GGTCACC G^GT[ACGT]ACC
BstPI 582 GGTCACC G^GT[ACGT]ACC
BstXI 404 CCAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 591 GCTTAGC GC^T[ACGT]AGC
DpnII 600 GATC ^GATC
Eco47III 36 AGCGCT AGC^GCT
EcoO65I 582 GGTCACC G^GT[ACGT]ACC
FbaI 599 TGATCA T^GATCA
FspI 158 TGCGCA TGC^GCA
HincII 142 GTCAAC GT[CT]^[GA]AC
HindII 142 GTCAAC GT[CT]^[GA]AC
HinfI 64 GATTC G^A[ACGT]TC
KspI 258 CCGCGG CCGC^GG
MaeIII 583 GTCAC ^GT[ACGT]AC
MboI 600 GATC ^GATC
MboII 417 GAAGA GAAGA(8/7)
MslI 537 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 600 GATC ^GATC
PflMI 387 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 472 CACGTG CAC^GTG
PmlI 472 CACGTG CAC^GTG
PpuMI 626 AGGACCT [GA]G^G[AT]CC[CT]
SacI 550 GAGCTC GAGCT^C
SacII 258 CCGCGG CCGC^GG
Sau3AI 600 GATC ^GATC
TaqI 344 TCGA T^CGA
TfiI 64 GATTC G^A[AT]TC
Tsp45I 583 GTCAC ^GT[GC]AC
TthHB8I 344 TCGA T^CGA
Van91I 387 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 348 GTAC GT^AC
525 GTAC
AvaI 463 CCCGAG C^[CT]CG[GA]G
622 CCCGAG
BanII 550 GAGCTC G[GA]GC[CT]^C
618 GGGCCC
BsgI 196 GTGCAG GTGCAG(16/14)
513 GTGCAG
BsoBI 463 CCCGAG C^[CT]CG[GA]G
622 CCCGAG
BspHI 323 TCATGA T^CATGA
542 TCATGA
DsaI 74 CCACGG C^C[GA][CT]GG
258 CCGCGG
NlaIII 324 CATG CATG^
543 CATG
PstI 289 CTGCAG CTGCA^G
670 CTGCAG
RcaI 323 TCATGA T^CATGA
542 TCATGA
RsaI 348 GTAC GT^AC
525 GTAC
SfcI 289 CTGCAG C^T[GA][CT]AG
670 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BbvI BmyI BpmI BsaAI
BsaHI BsaJI BsiYI BslI Bsp1286I
BspMI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MnlI MspA1I MspI MvaI
MvnI MwoI NlaIV PvuII Sau96I
ScrFI SfaNI TseI