Restriction map for
pFN21AE2694
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AcsI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BciVI BglII BlnI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FseI
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII ScaI
SexAI SfoI SfuI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 682 GACGTC GACGT^C
AflII 2026 CTTAAG C^TTAAG
AflIII 2723 ACACGT A^C[GA][CT]GT
AgeI 2320 ACCGGT A^CCGGT
Aor51HI 36 AGCGCT AGC^GCT
BanI 3478 GGTGCC G^G[CT][GA]CC
BbsI 2961 GAAGAC GAAGAC(2/6)
BcgI 2149 CGAATCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 599 TGATCA T^GATCA
BfaI 1090 CTAG C^TAG
BfrI 2026 CTTAAG C^TTAAG
BpuAI 2961 GAAGAC GAAGAC(2/6)
BsiEI 2180 CGGCCG CG[GA][CT]^CG
BspDI 2917 ATCGAT AT^CGAT
BsrDI 2351 CATTGC GCAATG(2/0)
ClaI 2917 ATCGAT AT^CGAT
EagI 2180 CGGCCG C^GGCCG
EclXI 2180 CGGCCG C^GGCCG
Eco47III 36 AGCGCT AGC^GCT
Eco52I 2180 CGGCCG C^GGCCG
FbaI 599 TGATCA T^GATCA
HindIII 2536 AAGCTT A^AGCTT
MaeI 1090 CTAG C^TAG
NotI 2179 GCGGCCGC GC^GGCCGC
NspI 2159 GCATGC [GA]CATG^[CT]
PinAI 2320 ACCGGT A^CCGGT
SacI 550 GAGCTC GAGCT^C
SalI 796 GTCGAC G^TCGAC
SapI 1908 GAAGAGC GCTCTTC(1/4)
SbfI 3055 CCTGCAGG CCTGCA^GG
SfiI 1857 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2319 CACCGGTG C[GA]^CCGG[CT]G
SphI 2159 GCATGC GCATG^C
Sse8387I 3055 CCTGCAGG CCTGCA^GG
StuI 1392 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 796 GTCGAC GT^[AC][GT]AC
3148 GTAGAC
AspI 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3460 GACGCTGTC
AviII 158 TGCGCA TGC^GCA
1685 TGCGCA
BbvCI 354 CCTCAGC CCTCAGC(-5/-2)
3169 CCTCAGC
BlpI 591 GCTTAGC GC^T[ACGT]AGC
1640 GCTCAGC
Bpu1102I 591 GCTTAGC GC^T[ACGT]AGC
1640 GCTCAGC
BsaWI 2320 ACCGGT [AT]^CCGG[AT]
2786 ACCGGA
BsmI 991 GAATGC GAATGC(1/-1)
2068 GCATTC
BspHI 323 TCATGA T^CATGA
542 TCATGA
BsrFI 2320 ACCGGT [GA]^CCGG[CT]
3303 GCCGGT
BssSI 2047 CACGAG CACGAG(-5/-1)
952 CTCGTG
BstEII 582 GGTCACC G^GT[ACGT]ACC
3591 GGTGACC
BstPI 582 GGTCACC G^GT[ACGT]ACC
3591 GGTGACC
CelII 591 GCTTAGC GC^T[ACGT]AGC
1640 GCTCAGC
Cfr10I 2320 ACCGGT [GA]^CCGG[CT]
3303 GCCGGT
DraIII 838 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2404 CACCACGTG
EarI 1908 GAAGAG CTCTTC(1/4)
3691 GAAGAG
EcoO65I 582 GGTCACC G^GT[ACGT]ACC
3591 GGTGACC
FspI 158 TGCGCA TGC^GCA
1685 TGCGCA
HincII 142 GTCAAC GT[CT]^[GA]AC
796 GTCGAC
HindII 142 GTCAAC GT[CT]^[GA]AC
796 GTCGAC
Ksp632I 1908 GAAGAG CTCTTC(1/4)
3691 GAAGAG
KspI 258 CCGCGG CCGC^GG
2976 CCGCGG
MseI 1943 TTAA T^TAA
2027 TTAA
PflFI 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3460 GACGCTGTC
RcaI 323 TCATGA T^CATGA
542 TCATGA
SacII 258 CCGCGG CCGC^GG
2976 CCGCGG
SmlI 2026 CTTAAG C^T[CT][GA]AG
3340 CTCAAG
Tru9I 1943 TTAA T^TAA
2027 TTAA
Tsp509I 1940 AATT ^AATT
2289 AATT
Tth111I 2370 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3460 GACGCTGTC
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AvaI AvaII BalI BamHI BanII
BbrPI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI