Restriction map for
pFN21AE1631
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 364 GCGG CCGC(-3/-1)
AclI 376 AACGTT AA^CGTT
AluI 317 AGCT AG^CT
AlwI 417 GGATC GGATC(4/5)
AlwNI 141 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 329 GGTCC G^G[AT]CC
BbvI 224 GCAGC GCAGC(8/12)
BcnI 132 CCGGG CC^[GC]GG
BpmI 81 CTCCAG CTGGAG(16/14)
BseRI 162 GAGGAG GAGGAG(10/8)
BsmAI 165 GAGAC GTCTC(1/5)
BsmBI 165 GAGACG CGTCTC(1/5)
BsmFI 330 GTCCC GGGAC(10/14)
BspHI 158 TCATGA T^CATGA
BsrI 348 CCAGT ACTGG(1/-1)
BstEII 303 GGTCACC G^GT[ACGT]ACC
BstF5I 135 GGATG GGATG(2/0)
BstPI 303 GGTCACC G^GT[ACGT]ACC
Cac8I 314 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 222 GCGC GCG^C
DpnII 418 GATC ^GATC
EcoNI 66 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 303 GGTCACC G^GT[ACGT]ACC
FauI 364 GCGGG CCCGC(4/6)
Fnu4HI 224 GCAGC GC^[ACGT]GC
FokI 135 GGATG GGATG(9/13)
HapII 132 CCGG C^CGG
HhaI 222 GCGC GCG^C
HinP1I 222 GCGC G^CGC
HpaII 132 CCGG C^CGG
ItaI 224 GCAGC GC^[ACGT]GC
MaeII 377 ACGT A^CGT
MaeIII 304 GTCAC ^GT[ACGT]AC
MboI 418 GATC ^GATC
MslI 109 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 362 CAGCGG C[AC]G^C[GT]G
MspI 132 CCGG C^CGG
MwoI 215 GCAACAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 132 CCGGG CC^[GC]GG
NdeII 418 GATC ^GATC
PpuMI 328 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 376 AACGTT AA^CGTT
RcaI 158 TCATGA T^CATGA
Sau3AI 418 GATC ^GATC
TseI 224 GCAGC G^C[AT]GC
Tsp45I 304 GTCAC ^GT[GC]AC
Tsp509I 426 AATT ^AATT
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 87 GTAC GT^AC
351 GTAC
BbsI 31 GTCTTC GAAGAC(2/6)
291 GTCTTC
BpuAI 31 GTCTTC GAAGAC(2/6)
291 GTCTTC
BsiYI 66 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCACAGCGGGG
BslI 66 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCACAGCGGGG
DdeI 71 CTCAG C^T[ACGT]AG
277 CTAAG
DraII 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
328 GGGTCCC
EcoO109I 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
328 GGGTCCC
MboII 32 TCTTC GAAGA(8/7)
292 TCTTC
RsaI 87 GTAC GT^AC
351 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BsaJI BssKI BstNI Cfr13I EcoRII
HaeIII HphI MnlI MvaI NlaIII
NlaIV Sau96I ScrFI SfaNI